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《China Biotechnology》 2009-01
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Nucleic Acid Sequence-Based Amplification and Its Applications in Viral Diagnosis

GAO Shan-dian CHANG Hui-yun CONG Guo-zheng DU Jun-zheng SHAO Jun-jun LIN Tong(Key Laboratory of Animal Virology of Ministry of Agriculture,State Key Laboratory of Veterinary Etiological Biology,National FMD Reference Laboratory,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China)  
Nucleic acid sequence-based amplification(NASBA) is a sensitive,isothermal,transcription-based amplification system specifically designed for the detection of RNA targets,which could amplify templete RNA in 2h under isothermal condition at about 42°C and without any special equipment.NASBA is now widely applicated in diagnosis of many pathogenic microorganism.It is mainly about principles and applications of NASBA in viral diagnosis.
【Fund】: 国家“十一五”科技支撑计划资助项目(2006BAD06A14 2006BAD06A17)
【CateGory Index】: R450
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