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《China Biotechnology》 2009-01
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Research Progress on Determination of Cellulase Activity and Gene Expression by Biosensor

YIN Juan YUAN Xing-zhong TANG Lin(College of Environmental Science and Engineering,Hunan University,Changsha 410082,China)  
Cellulase system contains a series of complex components.There are still some problems remained unclear in cellulase and its mechanism of hydrolyzing lignocellulosic materials and its hydrolysis kinetics,so profound study is needed.The rapid development of many kinds of new interdiscipline such as biochemistry,molecular biology and gene engineering,has further clarified the structure and function of cellulase,and the relationshi Pof its gene expression and regulation,and furthermore resulted in derivative study methods about cellulase in more aspects.Cellulase system components according to synergistic catalytic mode and the sequence of the homology amino acids similarity,summarizes traditional detection methods of enzyme components,and emphasizes on research progress of various biosensors applied in detection of cellulase activity and gene expression was introduced.
【Fund】: 国家自然科学基金(50678062);; 国家自然科学基金青年科学基金(50608029)资助项目
【CateGory Index】: Q819
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