Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《China Biotechnology》 2009-07
Add to Favorite Get Latest Update

The Construct of Myostatin Gene Targeted Recipient Myoblast Cell

ZHANG Lei1 YANG Xing-yuan2 AN Xiao-rong2 CHEN Yong-fu2(1 Liaoning Medical University,Jinzhou 121000,China)(2 State Key Laboratory for Agrobiotechnology,College of Biology,China Agricultural University,Beijing 100094,China)  
Myostatin(MSTN),a member of the transforming growth factor-β(TGF-β)superfamily,has been shown to be a negative regulator of myogenesis.Natural mutation in beef cattle causes double-muscling phenotypes.An investigation designed to knock out the myostatin gene by gene targeting in ovine myoblast cells were reported.Two promoter-trap targeting vectors MSTN-GFP and MSTN-Neo were constructed and used to transfect fetal and neonatal ovine primary myoblast cells.Both GFP expressing cells and drug resistant cells were obtained.Targeted cells expressing GFP were confirmed by PCR assay and drug resistant cells were characterized by PCR,Southern blot and DNA sequnce detection after growing into cell clones.That would supply nuleus transplantation donator for myostatin gene targetted somatic cloning sheep.
【Fund】: 国家“863”计划资助项目(2002AA206311)
【CateGory Index】: Q78
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved