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《China Biotechnology》 2009-07
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Functional Expression of Human Cytochrome P450 Enzymes in Escherichia coli

HAO Da-cheng1,2 XIAO Pei-gen2(1 College of Environmental and Chemical Engineering,Dalian Jiaotong University,Dalian 116028,China)(2 Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China)  
Functional expression of human cytochrome P450(P450,CYP)in E.coli is important for new drug RD,clinical drug therapy and the study of early ADME/T properties.Escherichia coli is the most extensively utilized host in the production of recombinant human P450 enzymes.However,it is challenging to obtain sufficient P450s with catalytic activity.Depict current developments in the heterologous expression of human P450 enzymes,strategies for the expression of human P450s in E.coli,factors affecting high-level expression,and coexpression.The recent works of authors in expression optimization are also reviewed.
【Fund】: 中国博士后科学基金(20080440019);; 辽宁省教育厅科研项目(2009A120)资助项目
【CateGory Index】: Q786
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