Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《China Biotechnology》 2009-08
Add to Favorite Get Latest Update

Construction and Expression of sGLP-1-HSA Fusion Protein

GAO Hua1 XU Chong-bo1 LI Yuan2(1 Key Laboratory of Bio-organic Chemistry of Liaoning Province,College of Medicine,Dalian University,Dalian 116622,China)(2 Dalian D.N Bio-engineering CO.,LTD,Dalian 116600,China)  
To reduce the clearance of sGLP-1,sGLP-1-HSA fusion protein was constructed by the fusion of the C-terminus of sGLP-1 to the N-terminus of HSA via an 6 amino acids linker and expressed in the Pichia pastoris.Screen the strain with resistsnce of 4g/L G418.After fermentation in a 5L bioreactor,the expression of sGLP-1-HSA was induced by methanol.After 48h,the expression level reached 0.8g/L.The sGLP-1-HSA was abtained following a series of purification steps such as ultrafiltration,sephadex G-25 and blue-sepharose.The purity of the fermentation product could reach 95% by means of HPLC,and the total recovery yield could reach 60%.The biological activity of sGLP-1-HSA in GKII diabetes rat and healthy rat suggested that the plasma glucose level was significantly lower after subcutaneous administration of sGLP-1-HSA..Expression of sGLP-1-HSA fusion protein can provide foudation for abtaining a larger quantity of recombinant sGLP-1-HSA for experimented and clinic studies.
【Fund】: 国家“十一五”计划“科技重大专项”(2009ZX09102-53)资助项目
【CateGory Index】: Q57
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 2 Hits
1 LIU Yan-li 1 LIU Yong-dong 2 WANG Yan-hui 1 WANG Yun-shan 2 SU Zhi-guo 2 MA Run-yu 1 (1. The Key Laboratory of Science and Technology of Controllable Chemical Reactions, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029; 2. The State Key Lab of Biochemical Engineering, the Institute of Process Engineering of the Chinese Academy of Sciences, Beijing 100080, China);High density culture conditions of recombinant human serum album from Pichia pastoris[J];Journal of Beijing University of Chemical Technology;2003-04
2 XIA Jie1*,XU Diansheng1,LU Bing1,AN Mi1,ZHANG Hua22.Changchun Qilong Biological Technology Research Institute, Changchun 130022, China);Fermentation Condition for the Expression of Human Lysozyme by Pichia pastoris SMD1168[J];Journal of East China University of Science and Technology;2003-04
Chinese Journal Full-text Database 3 Hits
1 DONG Peng CHEN Jin-chun LENG Xue(College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029);Studies of the growth kinetics and induction strategy with a strain of recombinant yeast Pichia Pastoris expressing the human insulin gene[J];Journal of Beijing University of Chemical Technology(Natural Science Edition);2006-03
2 SUN Chun-yi LI yuan LI Bie-hu HUANG Qing-sheng (Dalian D.N Bioengineering Company.Ltd,Dalian Liaoning 116600,China);Purification of GLP-1/albumin Expressed in Pichia pastoris[J];Journal of Dalian Nationalities University;2007-05
3 FENG Jing,XIA Jie,LU Bing,XU Dian-sheng(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China);Separation of Human Lysozyme from Fermentation Broth of Pichia pastoris by Aqueous Two-Phase Extraction[J];Journal of East China University of Science and Technology(Natural Science Edition);2006-12
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 QIU Rong De, LI Shi Yun, CHEN Jun Gang, WU Xiang Fu, YUAN Zhong Yi ( Shanghai Institute of Biochemistry, the Chinese Academy of Science, Shanghai 200031, China );High Expression and Purification of Recombinant Human Serum Albumin from Pichia pastoris[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;2000-01
2 Guo Meijing\ Chu Ju\ Hang Haifeng\ Zhuang Yingping\ Ye Qin\ Zhang Siliang (The State Key Lab of Bioreactor Engineering,East China University of Science & Technology 200237);Research Progresses in the Expression of Recombinant Human Serum Albumin[J];PROGRESS IN BIOTECHNOLOGY;2000-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved