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《China Biotechnology》 2009-08
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Cloning,Sequencing Analysis and Prokaryotic Expression of the IGF-Ⅰ Gene from Mink

RONG Min1 YANG Fu-he1 XU Shang-zhong2 WANG Gui-wu1 XING Xiu-mei1 WU Qiong1(1 The Institute of Special Economic Animals and Plants,Chinese Academy of Agricultural Sciences,Jilin 132109,China)(2 Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100094,China)  
The sequence of mink IGF-Ⅰgene was obtained and analyzed,and the fusion protein of the IGF-Ⅰ was produced in E.coli.By RT-PCR,liver IGF-Ⅰfrom mink were isolated.The IGF-ⅠcDNAs encoding 153 amino acids,and showed high degree of homology(90%)with other reported sequences(gold monkey,lesser panda,giant panda,A.tiger,horse,et al.)at nucleotide and amino acids levels.Two differences in mink amino acids sequences were found from sequence alignment.Whether these differences affect the molecular conformation or function for the growth factor's processing or these changes in the molecular structure may be responsible for the abnormal ability of growth and reproductive by influencing the function of IGF-Ⅰ have not yet been determined.The DNA fragment encoding the mature peptide of mink IGF-Ⅰwere subcloned to the pGEX-6P-1 expression vector and highly expressed in E.coli.BL21 with IPTG induction.The expression fusion proteins(pGEX-6P-1-IGF-Ⅰ)were mostly existed in soluble form and were about 34kDa.The induced cells culture were proved to be active IGF-Ⅰantigens.The mink IGF-Ⅰ gene was successfully cloned and expressed.Furthermore,the structure and function of this gene were analyzed and predicted.These result provided a good basis for further study of its biological activity.
【CateGory Index】: Q78
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 HUA Yi Min LIN Hao Ran  (Institute of Aquatic Economic Animal, School of Life Science, Zhongshan University, Guangzhou?510275, China);CLONING AND EXPRESSION IN PROKARYOTE OF GRASS CARP IGF-Ⅰ cDNA[J];Acta Zoologica Sinica;2001-03
【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 ZHANG Wei Min ZHANG Li Hong (School of Life Sciences, Zhongshan University, Guangzhou 510275, Chin a);Fusion expression of rainbow trout (Oncorhyncus mykiss) insulin-like growth factors Ⅰ and Ⅱ and their mitogenic activities in E. coli[J];Acta Zoologica Sinica;2003-02
2 ZHANG Li HUANG Xi-Gui WANG De-Shou(School of Life Science, Southwest China Normal University,Key Laboratory of Aquatic Science and Techonology, Chongqing 400715, China);Progress in the Studies on the IGF System in Fishes[J];Chinese Journal of Zoology;2005-02
3 LIU Jun,CHEN Ai-jing,HUANG Feng,ZHAO Sheng-jun,HU Bing,HOU Yong-qing(Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University,Wuhan 430023);Molecular Cloning and Sequence Analysis of Insulin-like Growth Factor Ⅰ cDNA from Carassius auratus[J];Freshwater Fisheries;2007-04
4 YE Xing, BAI Jun jie, LAO Hai hua, JIAN Qing, LI Ying hua, LUO Jian ren (Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, China);Fusion expression and purification of Ctenopharyngodon idellus IGF-I and preparation of the antiserum against GST-IGF[J];Journal of Fisheries of China;2002-02
5 ZHANG Dian-chang (South China Sea Fisheries Research Institute, CAFS, Guangzhou 510300, China);Research progress of insulin-like growth factors of fish[J];Journal of Shanghai Fisheries University;2005-01
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7 TONG Fu-dan, LIU Hong-yun (College of Animal Sciences, Zhejiang University, Hangzhou 310029);Molecular Cloning and Sequence Analysis of IGF-I from Triangular Bream (Megalobrama terminalis)[J];Scientia Agricultura Sinica;2004-10
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 LI Wen Sheng LIN Hao Ran (School of Life Sciences, Zhongshan University, Guangzhou 510275, China);EFFECTS OF SEX STEROID ON THE GROWTH HORMONE (GH) GENE EXPRESSION IN THE PITUITARY OF COMMON CARP ( CYPRINUS CARPIO)[J];ACTA ZOOLOGICA SINICA;2000-02
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