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《China Biotechnology》 2009-08
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Cloning and Bioinformatics Analysis of Fgf9,a Novel Gene Related to Sex Determination in Cow

LIAO Bing1 WU Ning1 HAN Feng-tong2 LIN Xiu-kun1(1 Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100094,China)(2 Institute of Oceanology,Chinese Academy of Science,Qingdao 266071,China)(3 Northeast Agricultural University,Harbin 150030,China)  
Fgf9 is the most important signal factor implicated in mediating the proliferation of Sertoli cells and the cord formation in testis morphogenesis of vertebrate.With the cloning principle of expressed sequence tags,sequence splicing and RT-PCR were applied in the cloning of novel gene from Holstein cow and analyzing its expression pattern in various cow tissues.The bioinformatics analysis on the sequence and protein results of Fgf9 was conducted.Results indicated that Fgf9 was located at cow 12 chromosome,with full-sequence of 697bp.The open reading frame is 627bp in length and encodes a deduced amino acid sequence of 208 residues.The molecular weight of Fgf9 protein is 23.38245kDa,and its pI is 7.0600.RT-PCR demonstrated the correctness of its ORF.Fgf9 was expressed in each tissue.No obvious homology with other cow cDNA was found for Fgf9.The GenBank accession number EU693028 was achieved.Function and structural analysis indicated that Fgf9 has a typical FGFs conserved domains,receptor interaction site and heparin binding site.It was predicted that Fgf9 has no signal peptide.
【Fund】: 国家自然科学基金资助项目(30671501)
【CateGory Index】: S823;Q78
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