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《China Biotechnology》 2009-08
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Structure Analysis of P8 Protein of Rice Gall Dwarf Virus and Its Expression in E. coli

GAO Fang-luan1 FAN Guo-cheng1,2 SHEN Jian-guo1,3 XIE Li-yan1LIN Qi-ying1 WU Zu-jian1 XIE Lian-hui1(1 Key Lab for Plant Virology of Fujian Province,Institute of Plant Virology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)(2 Research Institute of Pomology,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China)(3 Fujian Exit-Entry Inspection and Quarantine Bureau,Fuzhou 350001,China)  
To elucidate structural characters and expression characters of RGDV P8 protein in E.coli,bioinformatics analysis were performed by using biology software and analysis tools online.P8 gene was successfully amplified by RT-PCR and inserted to pGEX-4T-1 expression vector.The protein was then expressed in E.coli BL21(DE3)with IPTG induction.The antiserum was produced by immunizing rabbit with fusion protein,and the specificity of antiserum was evaluated by detecting RGDV,RDV,RSV,RRSV.Analysis showed that P8 protein had a typical transmembrane helices region,and a Phytoreo P8 domain shared with Phytoreo P8 family.SDS-PAGE and Western blotting analysis showed that molecular weight of P8 fusion protein was about 73 kDa.P8 protein was efficiently expressed in the form of inclusion body.The antiserum with high titer and specificity provides the basis for detecting RGDV and studying function of P8 protein.
【Fund】: 2007年公益性行业(农业)科研专项(nyzx07-051);; 福建省自然科学基金计划(2006J0065)资助项目
【CateGory Index】: S435.111.4
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【Citations】
Chinese Journal Full-text Database 4 Hits
1 FAN Guo-Cheng1, 2 WU Zu-Jian1** LIN Qi-Ying1 XIE Lian-Hui1(1. Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2. Pomology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China);Complete Sequence Determination and Structure Analysis of Genome Segment S8 of Rice gall dwarf virus[J];Journal of Agricultural Biotechnology;2005-05
2 Faan Hweichung;Zhang Shuguang;He Xianzhi South China Agricultural College Xie Shuangda;Liu Chaozhing;Zhou Lianggao Institute of Plant Protection,Guangdong Academy of Agricultural Sciences Liu Xianrong;Zhu Dong Guangdong Province Agricultural Bureau and Zhan Jiang District Agriculture Bureau;RICE GALL DWARF——A NEW VIRUS DISEASE EPIDEMIC IN THE WEST OF GUANGDONG PROVINCE OF SOUTH CHINA[J];Acta Phytopathologica Sinica;1983-04
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4 ZHANG Ting-ting,YE Bo-ping(School of Life Science and Technology,China Pharmaceutical University,Nanjing,210009,China);Progress in Study on Refolding and Renaturation of Inclusion Bodies[J];Pharmaceutical Biotechnology;2007-04
【Co-citations】
Chinese Journal Full-text Database 7 Hits
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5 FAN Guo-cheng~1,2,WU Zu-jian~1~(**),HUANG Ming-hua~3,LIAN Jun~3,LIANG Dong~3,LIN Qi-ying~1,XIE Lian-hui~1(1.Institute of Plant Virology,Fujian Agricultural and Forestry University,Fuzhou 350002,China;2.Pomology Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China;3.Station of the Monitoring and Forecasting of Crop Diseases and Pests Xinyi County,Guangdong 525300,China);Molecular characterization of the genome segment S9 of Rice gall dwarf virus[J];Virologica Sinica;2005-05
6 LIU Fu-xiu1,2, RUAN Xiao-lei1, HE Yun-wei1, LI Hua-ping1 (1Laboratory of Plant Virology, South China Agricultural University, Guangzhou 510642; 2 Post-Entry Quarantine Station for Tropical Plant, Hainan Entry-Exit Inspection and Quarantine Burean, Haikou 570311);Sequencing and Analysis of Rice Gall Dwarf Virus Genome Segment 6[J];Scientia Agricultura Sinica;2007-11
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