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《China Biotechnology》 2009-08
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The Activity Study of Aminodeoxychorismate Synthase of Differernt Corynebacterium glutamicum

REN Jian-hong1,2 ZHANG Xiao-mei1 DOU Wen-fang1 XU Hong-yu1 XU Zheng-hong1,2(1 Laboratory of Pharmaceutical Engineering,School of Medicine and Pharmaceutics,Jiangnan University,Wuxi 214122,China)(2 Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China)  
The two pabAB genes encoding aminodeoxychorismate synthase(ADC synthase)from a L-serine producing strain Corynebacterium glutamicum SYPS-062 and model strain Corynebacterium glutamicum ATCC 13032 were ampilified by PCR.The result of nucleotide sequence analysis showed that both pabAB fragments were 1863bp,encoding 620 amino acids.16 bases differences that resulted in the changes of 7 amino acids were found in the pabAB of SYPS-062.The two pabAB were inserted into pET-28a to yield the recombinant expression vector pET-28a-pabAB and then transfromed into BL21(DE3).Upon IPTG induction,soluble ADC synthase was over-produced by E.coli BL21(DE3)harboring the expression construct.Recombinant ADC synthase purified by Ni-NTA affinity chromatography showed a single band about 67kDa on SDS-PAGE gel,and activity of aminodeoxychorismate synthase analysis show that the enzyme specific activity of SYPS-062 is 46.6% lower than ATCC 13032.
【Fund】: 国家“863”计划(2007AA02Z207);; 国家“973”重点项目(2007CB707804);; 教育部新世纪优秀人才支持计划(NCET-07-0380)资助项目
【CateGory Index】: Q55
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 Chai Duoli,Qi Qiujing,Ji Muge,Zhang Liqiong (Hefei University of Technology,Anhui Hefei 230009,China);Study on the advance of serine[J];Chemical Technology Market;2006-05
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【Co-citations】
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【Secondary Citations】
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