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《China Biotechnology》 2009-08
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Study on the High Yield Strain of Gentamicin Producer by Protoplast Fused and Fermentor Test

GUAN Yu-xia LAN Ji-xian YAN Guang-xing ZHENG Jing-shi(College of Biological Science and Technology,Fuzhou University,Fuzhou 350002,China)  
The protoplasts of Micromonospora purpurea,the high yield strains of gentammicin producer were mutagenized by diethyl sulfate(DES)and ultraviolet radiation(UV)respectively,then fused,screened by gentamicin resistance and regenerated.The average fermentation unit 2200±U/ml could be achieved by shake flask for 10 batches.The average fermentation unit 1900±U/ml could be obtained by 5L fermentor for 7 batches.The quality of the end product conformed to CP2000,BP2000 and USP26 pharmacopoeia.
【CateGory Index】: TQ465
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【Citations】
Chinese Journal Full-text Database 5 Hits
1 LI He-di,JIN Zhi-hua,ZHANG Hai-guang,JIN hua (Department of Biological & Pharmaceutical Engineering,Ningbo Institute of Technology,Zhejiang University,Ningbo 315100);Protoplast formation,regeneration and UV mutagenesis of natamycin producing Streptomyces gilvosporeus[J];Industrial Microbiology;2008-03
2 LI Jinguo,JIANG Xingcun,DUANLiusheng.Space Medicine & Medical Engineering. 1995,8(2):113~116.;Mutagen Effect of Space Flight on Micromospra Echinopora[J];SPACE MEDICINE & MEDICAL ENGINEERING;1995-02
3 GUAN Yu-xia LIU Shu-tao(College of Biological Science and Technology,Fuzhou University,Fuzhou 350002,China);Effect of Some Amino Acids in Gentamicin Production[J];China Biotechnology;2007-12
4 GUAN Yu-xia,LIU Zhi-qiongCollege of Biological Science and Technology,Fuzhou University,Fuzhou 350002,China;Isolation and Identification of the Plasmid DNA in Three Micromonospora Strains Used in Production and Investigation of Their Functions[J];Journal of Southwest University(Natural Science Edition);2008-02
5 Zhao Min;Xie Fenglong;Wang Jie;Yang Xiaowei and Fan Jin (Jiangsu Institute of Microbiology,Wuxi 214063);Studies on formation ,regeneration and fusion of protoplast in Micromonospora echinospora var.producer of micronomicin[J];CHINESE JOURNAL OF NATIBIOTICS;1995-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Qiang Hua 1, Chen Wenlie 2, Zhong Xiurong 2, et al (1.Departement of Microbiology,\ 2.Laboratory of Electron Microscopy, Fujian Medical University, Fuzhou, 350004);Observations on the Formation and Release of the Protoplasts from Streptomyces hygroscopicus FC904[J];JOURNAL OF FUJIAN MEDICAL UNIVERSITY;1999-01
2 ZHANG Ling-hua~(1,2),TIAN Xing-shan~2,ZHOU Feng-zhen~2(1.College of Life Sciences,South China Agricultural University,Guangzhou 510640,China;2.Bio-Tech Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China);Effect of Space Flight on Physiological Index and Purification of Water of Bacterium Rhodopseudomonas capsulata[J];Fisheries Science;2006-02
3 HUANG Lei et al(Shenyang Agricultural University,Shenyang,Liaoning 110161);Research Progress of Space-biology[J];Journal of Anhui Agricultural Sciences;2005-09
4 Xie Qiong Li Yong-zhi Su Shuang-ning,et al.Institute of Space Medico-Engineering,Beijing 100094;Effects of space flight on biological characteristics of 13 microorganisms[J];Infection,Inflammation,Repair;2003-04
5 WANG Yan-fang et al(College of Life Science,Ludong University,Yantai,Shandong 264025);Progress in Space Mutation Microorganism[J];Journal of Anhui Agricultural Sciences;2009-16
6 ZHANG Ling-hua+1,2TIAN Xing-shan2* (1.South China University of Technology, Biological Engineering Institute, Guangzhou,Guangdong, 510640; 2^Bio-Tech Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong, 510640);STATUS IN SPACE MICROORGANISM MUTATION BREEDING[J];Acta Agriculturae Nucleatae Sinica;2004-04
7 YIN Hong, XIE Shen yi,ZHANG Guang ming, XIE Shen meng. Space Biology Lab,Chinese Acaderny of Space Technolgy Beijing,100086,China;Effect of Space Flight on Yield of Monascus Purpureus[J];Space Medicine & Medical Engineering;2003-05
8 WANG Fu zhuan, LIANG Qiu xia, LI Zong wei, WANG Yan ping, WU Jian (Henan Provincial Key Lab of Ion Beam Bioengineering Lab, Zhengzhou University, Zhengzhou, 450052);Applications of Mutation and Screening in Microbiology Breeding[J];Journal of Luoyang Teachers College;2002-02
9 JIA Xiao-jing (NCPC New Drug Research and Development Co.Ltd.,Shijiazhuang Hebei,050015,China);Application and progress of mutation breeding method of microbial drugs producing strain[J];Journal of the Hebei Academy of Sciences;2006-01
10 SHEN Hui-fang ZHANG Xiao-yong PU Xiao-ming LIN Bi-run (Institute of Plant Protection,Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong 510640);SCREENING HIGH-PRODUCING WANLONGMYCIN STRAINS BY SPACE MUTAGENSIS[J];Journal of Nuclear Agricultural Sciences;2009-04
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 CHENG Ji1, HONG Wen-rong1, SU Jian-zhang1, YE Li-rong2 (1. College of Qiaoxing Light Industry, Fuzhou University, Fuzhou, Fujian 350002, China; 2. Fuzhou Antibiotic General Factory, Fuzhou, Fujian 350002, China);Study on protoplast fusion between Streptomyces tenebrarius and Streptomyces kanamyceticus[J];Journal of Fuzhou University(Natural Sciences Edtion);2003-01
2 Liu Shi-ru(Central Laboratory,Kaifeng Pharmaceutical Plant, Kaifeng);STUDIES ON THE CONVERSION MECHANISM OF THE GENTAMICIN COMPONENTS[J];Chinese Journal of Antibiotics;1983-01
3 Zhao Min, Fan Jin, Bo Jian-xin Bu Hua-xiang(Jiangsu Province Research Institute of Microbiology, Wuxi);STUDIES ON THE SAGAMICIN PRODUCED BY MICROMONOSPORA ECHINOSPORA MUTANT STRAIN——I. TAXONOMICAL IDENTIFICATION OF M.ECHINOSPORA JIM-401[J];Chinese Journal of Antibiotics;1983-05
4 LUO Jian-mei, JIN Zhi-hua, CEN Pei-lin (Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China);Effects of cultivation conditions on natamycin production by Streptomyces gilvosporeus[J];Journal of Zhejiang University(Engineering Science);2005-02
5 Luo Jian-mei~1,2 , Jin Zhi-hua~1,2 and Cen Pei-lin1 (1 Department of Chemical and Biochemical Engineering, Zhejiang University, Hanghzhou 310027; 2 Department of Biological and Pharmaceutical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100);Determination of natamycin in fermentation broth by RP-HPLC[J];Chinese Journal of Antibiotics;2004-11
6 Zhao Min, Fan Jin and Bo Jian-xin (Jiangsu Institute of Microbiology, Wuxi);STUDIES ON SAGAMICIN PRODUCED BY M.ECHINOSPORA MUTANT STRAIN II. SELECTION AND FERMENTATION OF M.ECHINOSPORA JIM-401[J];Chinese Journal of Antibiotics;1984-02
7 Fan Jin and Zhao Min (Jiangsu Institute of Microbiology, Wuxi);STUDIES ON SAGAMICIN PRODUCED BY M. ECHINOSPORA MUTANT STRAIN Ⅲ. ISOLATION, PHYSICOCHEMICAL AND BIOLOGICAL PROPERTIES OF ANTIBIOTCS 401-B[J];Chinese Journal of Antibiotics;1984-02
8 CHEN Gui-Bin NIU Jin-Yang ZHANG Jian-Yon g YUAN Yin-Jin HU Zong-Ding (Department of Pharmaceutical Engineering, School of Chemical Engineering, Tianjin University, Tianjin 300072);STUDY ON BIOSYNTHESIS PATHWAYS OF A NTIB IOTIC AGPM[J];Microbiology;2003-05
9 ZHOU Li-Hong,LI Guo-Qin,WANG Zheng-Xiang,ZHUGE Jian (Research Center of Industial Microbiology The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 214036,China);Preparation and Regeneration of Protoplasts from Monascus purpureus and Genetic Transformation System[J];Hereditas(Beijing);2005-03
10 Liu Feng1, Fu Yi2 and Yan Hui-can2 (1 Bioengineering College, Fujian Teacher′s University, Fuzhou 350007; 2 Fuzhou Antibiotic Group Corp., Fuzhou 350002);Screening the high-yield mutants of gentamicin by 12C ion beam radiation and their productivity in fermentation tank[J];Chinese Journal of Antibiotics;2003-09
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