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《China Biotechnology》 2009-08
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The Mutasynthetic Production of CHC-B1 from a Streptomyces avermitilis Mutant in the Presence of Cyclohexanecarboxylic Acid

JIANG Wei1,2 WANG Yang2 ZHANG Xiao-lin2 GUO Wei-qun2 LIU Di1(1 College of Life Science,Northeast Agricultural University,Harbin 150030,China)(2 Academy of State Administration of Grain,Beijing 100037,China)  
Gene deletion vector pLJ04(pKC1139∷bkdF+bkdH)was used to disrupt bkdFGH in Streptomyces avermitilis 76-02-e,an industrial producer of anthelmintic avermectin.The disruptants were confirmed by PCR.Shake flask experiment and HPLC analysis showed that the mutant lost the ability to produce avermectins.As it is expected,the mutant,named S.avermitilis bkd76-3,could restore the ability of producing avermectins when the feeding of methylbutyric acid and isobutyric acid to its fermentations was carried out.The addition of cyclohexanecarboxylic acid(CHC)into fermentations of the S.avermitilis bkd76-3 allowed for production of four components,two of which was confirmed as CHC-B1 and CHC-A2 by LC/MS analysis,respectively.
【Fund】: 国家“863”计划(2006AA10A208);; “十一五”国家科技支撑计划(2006BAD08B02)资助项目
【CateGory Index】: TQ465
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