Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《China Biotechnology》 2009-08
Add to Favorite Get Latest Update

The Mutasynthetic Production of CHC-B1 from a Streptomyces avermitilis Mutant in the Presence of Cyclohexanecarboxylic Acid

JIANG Wei1,2 WANG Yang2 ZHANG Xiao-lin2 GUO Wei-qun2 LIU Di1(1 College of Life Science,Northeast Agricultural University,Harbin 150030,China)(2 Academy of State Administration of Grain,Beijing 100037,China)  
Gene deletion vector pLJ04(pKC1139∷bkdF+bkdH)was used to disrupt bkdFGH in Streptomyces avermitilis 76-02-e,an industrial producer of anthelmintic avermectin.The disruptants were confirmed by PCR.Shake flask experiment and HPLC analysis showed that the mutant lost the ability to produce avermectins.As it is expected,the mutant,named S.avermitilis bkd76-3,could restore the ability of producing avermectins when the feeding of methylbutyric acid and isobutyric acid to its fermentations was carried out.The addition of cyclohexanecarboxylic acid(CHC)into fermentations of the S.avermitilis bkd76-3 allowed for production of four components,two of which was confirmed as CHC-B1 and CHC-A2 by LC/MS analysis,respectively.
【Fund】: 国家“863”计划(2006AA10A208);; “十一五”国家科技支撑计划(2006BAD08B02)资助项目
【CateGory Index】: TQ465
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Bai Bao-xing,Zhang Chun-yan,Tian Min and Zhu Hui(China National Pharmaceutical Group Corporation,Sichuan Industrial Institute of Antibiotics,Chengdu 610051);Review on mutational biosynthesis in microbial medicinal fields[J];Chinese Journal of Antibiotics;2007-02
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 GAO Yuhong1,2,GUO Zhaocheng3,LIU Fangyue3,GAO Yuge4,SONG Mingxin1(1.College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China;2.Heilongjiang Vocational College of Animal Husbandry and Veterinary Science,Shuangcheng Heilongjiang 150111,China;3.Harbin Pharmaceutical Group Biovaccine Co.Ltd.,Harbin 150069,China;4.Tianjin Shengshilai Science and Technology Co.Ltd.,Tianjin 301609,China);Research advancement and application of doramectin[J];Journal of Northeast Agricultural University;2009-04
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 ZHU Hao_Jun\+\{1\}\ LIANG Yun_Xiang\+ 1* \ ZHOU Jun_Chu\+1\ ZHENG Y ing_Hua\+2(\+1Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China) \ \+2(Beijing Institute of Biomedicine, Beijing 100091, China);Effects of bkdAB Interruption on Avermectin Biosynthesis[J];Chinese Journal of Biotechnology;2004-02
2 CHENG Shan, XIA Huan-zhang (School of Pharmaceutical Engineering, Shengyang Pharmaceutical University, Shengyang 110016, China);Advances in the biosynthesis genes of the aminoglycoside antibiotics[J];Journal of Shenyang Pharmaceutical University;2003-01
3 Fan Ming-qi1 and Zhao Min2(1 School of Biology,The University of Nottingham,NG 7 2RD,Notingham,UK;2 Jiangsu Institute of Microbiology,Wuxi 214063);The metabolite regulation of aminoglycosides biosynthesis and methods for improvement of aminoglycosides producing strains[J];Chinese Journal of Antibiotics;2005-09
4 ZHU Hao-Jun 1 LIANG Yun-Xiang 1* ZHOU Jun-Chu 1 ZHENG Ying-Hua 2 ( 1 Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China) ( 2 Beijing Institute of Biomedicine, Beijing 100091, China);Effects of bkdF Interruption on Avermectin Biosynthesis[J];Acta Microbiologica Sinica;2004-02
5 ZHANG Ji-yu, LI Jian-yong, ZHOU Xu-zheng, LI Jin-shan, ZHANG Mei, XU Zhong-zan, LI Hong-sheng, HU Jun-jie (Key Laboratory for New Animal Medicine Engineering, Ministry of Agriculture/ Lanzhou Institute of Animal Science and Veterinary Pharmaceutics, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China);Pharmacokinetics of Doramectin in Pigs[J];Chinese Journal of Animal and Veterinary Sciences;2005-07
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved