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《China Biotechnology》 2009-08
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Preliminary Research on Gold Nanoparticles Enhancing the Specificity of PCR Amplification of Low Copy Gene from the Complicated System

LI Hai-kuo1 CAO Xue-yan1 ZHOU Zhi-lei1 CHEN Heng2(1 Nanobiology Laboratory,Bio-X Life Science Research Center,College of Life Science and Biotechnology,Shanghai JiaoTong University,Shanghai 200240,China)(2 Shanghai Information Center for Life Sciences,Chinese Academy of Sciences,Shanghai 200031,China)  
Objective:Addition of gold nanoparticles(average diameter10nm)into amplification system with low copy gene fragments from the complicated genome helps to enhance the specificity of PCR amplification.Methods:PCR amplification model system of the complicated genome was simulated,PCR was performed with approximate single copy λ DNA as the template and addition of gold nanoparticles.The experiments were so repeated and optimized that the PCR amplification model system was built by simulating the PCR amplification of the complicated genome with low copy gene.Then,the low copy gene(tumor necrosis factor,TNF-α gene exon 1 with 380bp)of human genome was amplified so as to verify the actual efficiency of enhancement of the specificity of PCR amplification by the gold nanoparticles.Results:The gold nanoparticles could better enhance the specificity of PCR amplification in the PCR amplification of the complicated genome system with low copy gene.Conclusion:It was preliminarily indicated that nanoparticle PCR method based on gold nanoparticles could optimize the PCR amplification of the complicated actual genome system with low copy gene,which made an important valuable reference to improvement and extension of the optimized methods of PCR amplification.
【Fund】: 国家自然科学基金重点项目(10335070)资助项目
【CateGory Index】: Q78
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 WANG Wei +1 WANG Hai bin +2 LI Zhi xin +1 GUO Zeng yuan +1 (1 School of Aerospace,Tsinghua University Beijing 100084,China) (2 Department of Laboratory Medicine, No. 302 Hospital of PLA Beijing 100039,China);Studies of Silicon Inhibition Effects on Polymerase Chain Reaction[J];Progress In Biotechnology;2005-03
2 WANG Qun,LI Jing,CAO Xiao-hong,ZHANG Zhi-zhou(Teda Bio-X Center for Systems Biotechnology,College of Food Engineering and Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China);Silver Nanoparticles Enhance the Specificity of Repeated Long PCR Amplification[J];Journal of Tianjin University of Science & Technology;2007-02
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