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《China Biotechnology》 2009-08
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Study on Electrotransformation of vitro DNA into Pseudomonas putida

DENG Ting-ting1 LI Hui1 LIU Yan2 ZHENG Yuan-yuan2 LI Chun1,2(1 Agricultural College of Shihezi University/Key Laboratory for Green Processing of Xinjiang Bingtuan,Shihezi 832003,China)(2 School of Life Science and Technology,Beijing Institute of Technology,Beijing 100081,China)  
Pseudomonas putida isolated relieving salt stress and promoting plant growth was used as the recipient strain.Optimum conditions including growth stage of the strain,concentration of competent cell,electroshock voltage and the meduim were investigated for the electroporation of P.putida-E.coli shulter vector PDSK519 into P.putida Rs-198.It was showed that the highest transformation efficiency was up to 1.3×107CFU/μ g DNA in the optimium condition,under which the competent cells were collected at logarithmic growth phase(OD600=0.5),the mixture of the competent cells(4.6×1012/ml)and PDSK519 plasmid DNA was electroporated at 13kV/cm.It will be very helpful for developing a genetic transformation system and studying the genomic function of P.putida.
【Fund】: 国家自然科学基金(20776017);; 新疆维吾尔自治区高新技术重点项目(200811108)资助项目
【CateGory Index】: X172
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