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《China Biotechnology》 2009-08
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Cloning and Preliminary Analysis of the Stem Specific Promoter of Sugarcane

CAI Wen-wei WANG Zheng-peng ZHANG Shu-zhen YANG Ben-peng(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)  
Sugarcane stem is an ideal organ for producing foreign pharmaceutical proteins and chemicals by genetic engineering.A perfect promoter driving foreign gene to express strongly and specifically in sugarcane stem is necessary for this purpose.In order to isolate a Sugarcane stem-specific promoter,a fragment of 1968bp nucleotide sequence(Ppst2a)upstream 5' of sugarcane pst2a gene,which was demonstrated to express specifically in sugarcane stem previously was isolated by using chromosomal walking.Bioinformatical analysis of this sequence shows that the sequence contains some typical elements of a promoter.To identify the stem-specific of this promoter,a construct was derived from pCAMBIA1301,which original CaMV 35S promoter was replaced by the 1968bp nucleotide sequence,and named as pCAMBIA1900.Transformations of pCAMBIA1900 and pCAMBIA1301 to leaves and stem pieces of sugarcane were carried out by using particle bombardment.The transient expression of gus showed that the gus expressed specifically in sugarcane with a little higher level compared with CaMV 35S.It is the first report that pst2a promoter is a potential stem-specific promoter which can further be used in transgenes into sugarcane.
【Fund】: 国家自然科学基金(30660097);; 现代农业产业技术体系建设专项资金;; 中央级公益性科研院所基本科研业务费资助项目
【CateGory Index】: S566.1;Q943.2
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 YAO Wei* GENG Guang-liang YU Ai-li ZHANG Mu-qing CHEN Ru-kai*(Key Lab of Eco-physiology & Genetic Improvement for Sugarcane, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China);An Improved Method for Isolation of Transgenic Sugarcane Genomic DNA[J];Journal of Tropical and Subtropical Botany;2004-03
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 WANG Ji-hua,LI Yu-liang,HU Jian-guang,AN Kang,CAO Gan(Crop Research Institute Guangdong Academy of Agricultural Sciences,Key Laboratory of Crops Genetics & Improvement of Guangdong Province,Guangzhou 510640);A Method for Rapidly Extracting Genomic DNA from Transgenic Sugarcane[J];Sugarcane and Canesugar;2007-06
2 ZHU Ya-lan1,2,YAO Wei1,2,DOU Jian-hua1,2,YUE Chao-yin1,2,HE Zheng-quan1,2,CHEN Fa-ju1,2,LIANG Hong-wei1,2,LIANG Wei1,2 (1.Biotechnology Research Center,Three Gorges University,Yichang 443002,China;2.Key Laboratory of Natural Products Research and Development,Yichang 443002,China);Cloning and Analysis of Seeds Oil-body-specific Promoter from the Brassica napus and Construction of Plant Expression Vector[J];Acta Agriculturae Boreali-Sinica;2007-02
3 YU-Yun fang1,2,YAO Wei1,ZHANG Jin3,HE Zheng-quan1,Yue Chao-yin1,LIANG Hong-wei1;A Study on Producing C Reactive Protein by Transgene Technology[J];Acta Agriculturae Universitatis Jiangxiensis;2008-03
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Chinese Journal Full-text Database 2 Hits
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