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《China Biotechnology》 2009-08
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The Construction Strategy for Tandem Polypeptide Genes

ZHANG Jun YANG Ya-lin TIAN Zi-gang WANG Jian-hua(Key Laboratory of Feed Biotechnology of MOA,Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China)  
The tandem expression vector can effectively increase the expression level and structure stability of the target peptides(protein)with small size of molecular,and avoid toxicity towards host cell from some toxic peptides product,therefore,this approach is widely applied in biotechnology.The four construction methods of tandem expression plasmid including asymmetric and complementary cohesive ends,directional adapter,isocaudarners,and tandem expression cassette were reviewed in terms of protocol,characteristic and applicable field.In addition,selection principles from various construction methods of tandem plasmid were reviewed in terms of efficiency,accuracy and product cleavage.
【Fund】: 国家自然科学基金(30771574 30810303084);; 北京市自然科学基金(5062031 5093030)资助项目
【CateGory Index】: Q78
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