Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《China Biotechnology》 2009-08
Add to Favorite Get Latest Update

The Construction Strategy for Tandem Polypeptide Genes

ZHANG Jun YANG Ya-lin TIAN Zi-gang WANG Jian-hua(Key Laboratory of Feed Biotechnology of MOA,Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China)  
The tandem expression vector can effectively increase the expression level and structure stability of the target peptides(protein)with small size of molecular,and avoid toxicity towards host cell from some toxic peptides product,therefore,this approach is widely applied in biotechnology.The four construction methods of tandem expression plasmid including asymmetric and complementary cohesive ends,directional adapter,isocaudarners,and tandem expression cassette were reviewed in terms of protocol,characteristic and applicable field.In addition,selection principles from various construction methods of tandem plasmid were reviewed in terms of efficiency,accuracy and product cleavage.
【Fund】: 国家自然科学基金(30771574 30810303084);; 北京市自然科学基金(5062031 5093030)资助项目
【CateGory Index】: Q78
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 4 Hits
1 YU Guang Rong;SUN Xue Zheng;SHEN Wei Ying;HOU Li Li;FENG Wen Hua;JIANG Shu;SHEN Qing Xiang Shanghai Institute of Planned Parenthood Research, Shanghai 200032 Shanghai Medical College, Fudan University, Shanghai 200032;EXPRESSION OF HCGβ-CTP POLYMERIDE BY THE METHYLOTROPIC YEAST, PICHIA PASTORIS AND STRUCTURE PREDICTION OF THE EXPRESSED PRODUCTS[J];Acta Biologiae Experimentalis Sinica;2005-02
2 JING Shen-rong~△,ZOU Quan-ming,HONG Yu,et al(()~△Department of Clinical Microbiology and Immunology,Faculty of Medical Laboratory Science,Third Military Medical University,Chongqing 400038,China);Construction of Multiple Copy Expression Cassette for Expression of Human Interleukin-10 in Pichia pastoris[J];Chinese Journal of Biologicals;2007-02
3 WANG Xiao-Fu1,2,LIU Ren-Hu1,CHEN Xiao-Yun1,GAO Zhi-Mou2,CHEN Jin-Qing1 1. Institute of Virology and Biotechnology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;2. College of Life Sciences,Anhui Agricultural University,Hefei 230036,China;Construction of vectors for expression of cleavable tandem repeat Thanatin fusion protein in plants[J];Hereditas;2007-06
4 LIANG Wei-feng ZHANG Chao-chun YANG Xi-cai * (Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080,China);Construction of a multi-copy Pichia expression vector and expression of human brain-derived neurotrophic factor in Pichia pastoris[J];Acta Microbiologica Sinica;2005-01
Chinese Journal Full-text Database 9 Hits
1 BAO Xiao-lan, WANG He-ping, BAO li-gao, ZHOU PingLIN Ri-sheng,ZHAO Li-xia(College of Bioengineering,Inner Mongolia Agricultural University,Huhhot 010010,China);CONSTRUCTION OF RECOMBINANT CONSTITUTIVE EXPRESSION VECTOR FOR β-MANNANASE GENE[J];Journal of Inner Mongolia Agricultural University(Natural Science Edition);2007-04
2 HAN Gang1,2,GAO Jiang-ping2,YAN Jin-qi1,JIA Rui1,ZHANG Liang1,DONG Jin-kai1,2,TIAN Ren-li1,2,WANG Xiao-xiong2,YU Ji-yun1(1.Institute of Basic Medical Science,Academy of Military Medical Sciences,Beijing 100850,China;2.Department of Urology,Chinese PLA General Hospital,Beijing 100853,China);Construction and eukaryotic expression of DNA vaccine encoding heterological prostate stem cell antigen for prostate cancer[J];Academic Journal of PLA Postgraduate Medical School;2009-01
3 JIN Feng-Liang,ZHANG Cheng-Wen,XU Xiao-Xia,SUN Qiang,REN Shun-Xiang(College of Natural Resources and Environments,South China Agricultural University/Engineering Research Centre of Biological Control,Ministry of Education,Guangzhou 510642,China);High-level fusion expression of thanatin with an ubiqutin fusion partner in Escherichia coli[J];Acta Entomologica Sinica;2009-05
4 WANG Xiao-li1 LIU Dong2 LIANG Shi-zhong1(1 School of Bioscience and Bioengineering, South China University of Technology,Guangzhou 510640,China)(2 Shenzhen Polytechnic,Shenzhen 518055,China);Strategys of Recombinant Construction and High Expression for Antihypertensive Peptides[J];China Biotechnology;2008-03
5 Wen-hua FENG1,2, Yan SHI1,2, Guang-rong YU1,2, Wei-ying SHEN2, Shu JIANG2, Qing-xiang SHEN2 (1. Shanghai Medical College, Fudan University, Shanghai, 200032) (2. Shanghai Institute of Planned Parenthood Research, Shanghai, 200032);Expression of Fusion Protein hCGβ-CTP Polymer and 3C3d in Pichia pastoris Cells[J];Reproduction & Contraception;2006-05
6 HU Shi-jie,LUO Su-lan,ZHANG Ji-zhen,ZHANGSUN Dong-ting(Key Laboratory for Tropical Biological Resources,Ministry of Education;Ocean College,Center for Experimental Biotechnology,Hainan University,Haikou 570228,China);Pichia pastoris Expression System and Strategies for High-level Expression[J];Biotechnology;2007-06
7 Yan SHI~(1,2) Shu JIANG~2 Wei-ying SHEN~2 Ya-hong JIANG~2 Qing-xiang SHEN~2 (1.Shanghai Medical College,Fudan University,Shanghai,200032) (2.Shanghai Institute of Planned Parenthood Research,Shanghai,200032);Expression and Purification of the Fusion Proteins hCGβ-(CTP)_4 and hCGβ-(CTP)_4-(C3d-P28)_4 in E.coli[J];Reproduction & Contraception;2007-04
8 ZHU Ji~1 GAO Fei~2 YI Yu~1 CHEN Jian-shu YING Guo-qing~(1*) 1 College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310014,China; 2 ABON Biopharm (Hangzhou) Co.,Ltd,Hangzhou 310018,China;Progress on antimicrobial peptides[J];Chinese Bulletin of Life Sciences;2008-04
9 HAN Gang1,2, YAN Jin-Qi1, GAO Jiang-Ping2, JIA Rui1, ZHANG Liang1, DONG Jin-Kai1,2, TIAN Ren-Li1,2, WANG Xiao-Xiong2, LI Ren3, YU Ji-Yun1 1.Institute of Basic Medical Science, Academy of Military Medical Sciences, Beijing 100850;2.Department of Urology, the PLA General Hospital, Beijing 100853;3.Cancer Center of Chinese People's Armed Police Forces General Hospital, Beijing 100039;China;Construction and Eukaryotic Expression of a Series of Fusion Genetic Fragments Encoding Multi-Copies Heterological Prostate Stem Cell Antigen[J];Letters in Biotechnology;2009-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Li Ying1 Yu Xiaolin2 Li Naijian1* Wang Hengming1 Huang Ziran31 Vegetable Research Institute of Guangdong Academy of Agriculture Sciences, Guangzhou, 510640; 2 Institute of Vegetable Science in Zhejiang University, Hangzhou, 310029; 3 Department of Animal Science in South China Agricultural University, Guangzhou, 510642;Evaluation of Resistance to Bacterial Wilt and Systemic Breeding in Cecropin-GM Capsicum[J];Molecular Plant Breeding;2005-02
2 JianYuyu1  WuXingrong1  MoHaokue1  Chen Fengzhen1 Dong Chun2  Huang Ziran3(1 Genetic Engineering Laboratory, South China Agric. Univ., Guangzhou, 510642; 2 College of Natural Resources & Environment; 3 Dept. of Sericulture,South;TRANSFER OF CECROPIN B GENE INTO RICE (Oryza sativa L.) BY THE BIOLISTIC METHOD AND TRANSGENIC PROGENY LINE FOR RESISTANCE TO BACTERIAL BLIGHT[J];JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY;1997-04
3 DING Yun fei,LIU Yong,LIU Hong yan,MA Yan bing,SUN Qiang ming,SUN Mao sheng,DAI Chang bai * (Institute of Medical Biology, Peking Union Medical College, Kunming 650118, China);Expression of the Secretory Hepatitis B Virus Middle Surface Protein in Pichia pastoris[J];Chinese Journal of Biochemistry and Molecular Biology;2002-03
4 WT4BZ]WANG Jian, SHEN Wei ying, ZHOU Qing ping, SHEN Qing xiang [WT5”BZ] (Shanghai Institute of Planned Parenthood Research, Shanghai, 200032) [WT4HZ];Expression of the Gene Encoding the Tetraploid of Carboxyl terminal 37 Peptides of β-hCG in E.coli[J];REPRODUCTION AND CONTRACEPTION;2000-03
5 ZHOU Peng,GUO Anping,LI Xiaoying,Haikou 571101,Hainan,China);Reconstruction of pAO815 of Yeast Expression Vector by Overlap Extension[J];Life Science Research;2002-04
6 LI Ke qin 1) , LI Chun hai 1)* , YAN Chun hong 2) ( 1) Department of Tumor Molecular Biology,Affiliated Hospital to Academy of Medical Sciences, Beijing 100850,China; 2) Department of Cancer Biology,M.D.Anderson Cancer C;The Expression and Characterization of Human Tissue Inhibitor of Metalloproteinase-1 in Pichia pastoris Yeast System[J];Chinese Journal of Biochemistry and Molecular Biology;2001-02
7 SHEN Qing-Xiang; LIU Hai-Hu; LI Chang-Lin and JIN Wen-Jie(Shanghai Institute of Cell Biology, Academia Sinica,200031)REN Pei-Feng and GONG Yue-Ting(Shanghai Institute of Biochemistry,Academia Sinica,200031);Expression of β-Subunit of Human Chorionic Gonadotropin in Chinese Hamster Ovary Cells[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1995-02
8 Liu Qingzhong 1,Zhao Hongjun 1,Liu Peng 1,Mong Rengong 2,and Freddi A.Hammerschlag 3 ( 1Shandong Institute of pomology,Tai'an,Shandong 271000; 2USDA/ARS HCRL,Corvallis,OR 97330,USA; 3USDA/ARS,Fruit Laboratory,Beltsville,MD 20705,USA);Regeneration of Tetraploid Plants with Cecropin MB_(39) Gene from'Royal Gala'Apple[J];Acta Horticulturae Sinica;2001-05
9 DENG An Mei,ZHONG Ren Qian,CHEN Sun Xiao et al. Clinical Immunology Center,Changzheng Hospital, Shanghai 200003;Sequence analysis of autoantigen SS-B/La[J];CHINESE JOURNAL OF IMMUNOLOGY;2000-09
10 Jia Shirong1 Qu Xianming2 Feng Lanxiang3 Tang Ti4Tang Yixiong1 Liu Kun3 Zhao Yanli1 Bai Yongyan4 Cai Minying2(1 Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing 100081;2Shanghai Resea;Development of Potato Clones with Enhanced Resistance to Bacterial Wilt by Introducing Antibacterial Peptide Gene[J];SCIENTIA AGRICULTURA SINICA;1998-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved