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《China Biotechnology》 2009-08
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The New Technique for Protein Analyses:Proximity Ligation Assay and Its Application

TANG Na1 SHEN Zhi-qiang1,2 GUAN Yu1 WANG Jin-liang1(1 Key Laboratory of Preventive Veterinary Medicine and Animal Biotechnology,Binzhou Animal Husbandry andVeterinary Research Institute in Shandong,Binzhou 256600,China)(2 Lüdu Biological Technology Co.,Ltd,Binzhou 256600,China)  
Proximity ligation assay(PLA)is a recently developed strategy for protein analysis in which a pair of proximity probes are designed to bind pairwise to target proteins via a DNA ligation reaction of oligonucleotides results in the formation of an amplifiable DNA strand suitable for analysis.It allows extremely sensitive and specific detection of proteins by allowing the protein targets to be represented as DNA signatures that may be subsequently amplified and detected by methods like PCR.Here the basic principle and the potential applications of PLA were discussed,as well as the preparation of proximity probes.
【CateGory Index】: Q51
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