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《China Biotechnology》 2015-01
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Overexpression of Lc CHYB to Enhance the Tolerance to Oxidative Stress of Eustoma Grandiflorum

JIN Chao;LIU Jie;JI Jing;WANG Gang;CAO Hai-yan;WU Jiang;School of Environmental Science and Engineering,Tianjin University;School of Chemical Engineering and Technology,Tianjin University;  
Reactive Oxygen Species was accumulated when the plant was under the abiotic stress,which damaged the cell structure and proteins. Carotenoids are non-enzymatic antioxidants,playing an important role to enhance the tolerance to oxidative stress in plants. β-carotene hydroxylase was a key enzyme in carotenoid biosynthesis pathway and coded by CHYB gene. In this study,the Lc CHYB was overexpressed in transgenic Eustoma grandiflorum,the results shown When the plants were exposed to 10% H2O2,the Fv/ Fm,ФPSⅡ,SOD and POD increased significantly,the content of total carotenoids,Zeaxanthin and Xanthophyll cycle were also enhanced significantly in transgenic line compare to the wild type. Overexpression of Lc CHYB enhanced the tolerance to oxidative stress in Eustoma grandiflorum was proved.
【Fund】: 国家自然科学基金(31300329 31271793 31271419);; 国家转基因生物新品种培育科技重大专项(2014ZX08003-002B)资助项目
【CateGory Index】: Q945
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【Co-citations】
Chinese Journal Full-text Database 8 Hits
1 XIAO Lan,LAO Yong-min,JIANG Jian-guo(School of Bioscience and Bioengineering,South China University of Technology,Guangzhou 510006,China);Clone and Analysis ζ-Carotene Desaturase of Dunaliella bardawil[J];Modern Food Science and Technology;2012-07
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7 Ji Jing;Yang Hailan;Wang Gang;Wu Weidang;Jin Chao;Wu Jiang;School of Chemical Engineering and Technology,Tianjin University;Research Institute of Genetic Engineering,Tianjin University;;Effect of Over-Expression of AtchyB on the Oxidation Resistance of Transgenic Eustoma Grandiflorum[J];Journal of Tianjin University(Science and Technology);2014-08
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