Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《China Biotechnology》 2015-01
Add to Favorite Get Latest Update

Differentially Expressed Analysis on the Responsive Genes to Salt Stress in Sunflower by cDNA- AFLP

SUN Rui-fen;ZHANG Yan-fang;GUO Shu-chun;YU Hai-feng;LI Su-ping;QIAO Hui-lei;NIE Hui;AN Yu-lin;Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences;  
Objective: This study was conducted to elucidate the response of genes in sunflower to saltstress,isolate and identify the genes related to salt tolerance. Methods: The differentially expressed gene fragments produced after being treated with salt were analyzed by c DNA-AFLP. Results: 232 primer pairs obtaining differential expressed bands were acquired from 256 primers combinations. Using these primer pairs,a total of 845 differentially expressed up-regulated transcript-derived fragments( TDFs) were selectively amplified,42 positive TDFs from them were identified after the second PCR amplification and reverse Northern blot. 12 TDFs of which were cloned and sequenced,and 10 nucleotide sequences were obtained. By Blastx analysis,10 TDFs were tightly related to salt tolerence of sunflower,being involved in signal transduction related proteins,abiotic stress related functional proteins,senescene associated proteins and proteins associated with protein interaction. Conclusion: A number of genes that respond to salt stress were identified using c DNA-AFLP,the results will lay a foundation for determining potential molecular mechanisms of salt tolerance and the practice of molecular breeding of sunflower.
【Fund】: 内蒙古自治区自然科学基金资助项目(2012MS0315)
【CateGory Index】: S565.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 10 Hits
1 MA Hong-shuang1,2;XIA Xin-li1;YIN Wei-lun1.1 National Engineering Laboratory for Forest Tree Breeding,Beijing Forestry University,100083,P.R.China;2 Preparation Office of Taishan Botanical Garden,Taiyuan,030006,P.R.China.;Cloning and analysis of SCL7 gene from Populus euphratica[J];Journal of Beijing Forestry University;2011-01
2 Liu Dali1 Zhang Xinxin1, 2 Cheng Yuxiang1 Tetsuo Takano2 Liu Shenkui1*1 Stress Molecular Biology Laboratory, Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin, 150040; 2 Asian Natural Environmental Science Center, The University of Tokyo, Midori Chyou1-1-1, Tokyo, 1880002, Japan;Cloning and Characterization of the rHsp90 Gene in Rice (Oryza sativa. L) under Environmental Stress[J];Molecular Plant Breeding;2006-03
3 WANG Aiqin~1,WANG Zizhang~1,YANG Litao~1,WEi Yutuo~3,Li Yangrui~(1,2),(1Agricultural College,Guangxi University,Nanning 530005,China; 2Guangxi Academy of Agricultural Sciences,Nanning 530007,China3College of Life Science and Technology,Guangxi University,Nanning 530005,China);Research progress of two key enzyme genes on the ethylene biosynthesis[J];Journal of Guangxi Agricultural and Biological Science;2004-02
4 LI Gui-ying;YANG Cheng-jun;College of Forestry,Northeast Forestry University;;Research Situation of GRAS Family Transcription Factor in Plants[J];Journal of Anhui Agricultural Sciences;2014-14
5 CHEN Yin-hua~(1,2),HUANG Wei~3,WANG Hai~1(1.College of Life Science and Agriculture,Hainan University,Haikou 570228;2.College of Horticulture & Forestry Huazhong Agricultural University,Wuhan 430070; 3.Division of Teaching Administration,Hainan University,Haikou 570228,China);Review on Researching Advance in ACC Oxidase Genes[J];Natural Science Journal of Hainan University;2006-02
7 SHI Gong-yao1,WANG Yu-mei2,HUA Jin-ping1(1.College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193; 2.Institute of Cash Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,China);Aquaporins and Salt Tolerance of Higher Plants[J];Journal of Agricultural Science and Technology;2012-04
8 TANG Li1,2 TANG Hui3 KWAK Sang-Soo4 LEE Haeng-Soon4 WANG Su-ying1,2 YANG Xiao-li2 (1 Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin 300134, China)(2 College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China)(3 College of Bioscience and Biotechnology, HLJ August First Land Reclamation University, Daqing 163319, China)(4 Environmental Biotechnology Research Center, Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea);Improving Potato Plants Oxidative Stress and Salt Tolerance by Gene Transfer Both of Cu/Zn Superoxide Dismutase and Ascorbate Peroxidase[J];China Biotechnology;2008-03
9 LI Wen-Ke,MA Chun-Sen(State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China);Present properties, mechanism and prediction of antifreeze proteins[J];Chinese Bulletin of Life Sciences;2012-10
10 LIU Jinping(College of Agronomy/Ministry of Education Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources,Hainan University,Haikou,Hainan 570228);Advances in Research on Key Enzyme Genes of Ethylene Biosynthesis[J];Chinese Journal of Tropical Agriculture;2013-01
Chinese Journal Full-text Database 10 Hits
1 WANG Dan-dan et al (College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083);Establishment of a cDNA-AFLP System in Ammopiptanthus mongolicus and Primer Screening[J];Journal of Anhui Agricultural Sciences;2009-24
2 ZHANG Li,JIANG Chang-jun,XU Zhen-guo,YANG Shu-yun,YUAN Zhi-hong,DING Zhou(Key Laboratory of Tea Biochemistry and Biotechnology,Ministry of Agriculture,Anhui Agricultural University,Hefei 230036);Differential gene expression analysis of tea seeds induced by cold stress using cDNA-AFLP[J];Journal of Anhui Agricultural University;2008-03
3 HUANG Feng-lan1,2,XUE Gui-bin3,MENG Fan-juan1,YAN Xiu-feng1(1.College of Life Science,Northeast Forestry University,Harbin,Heilongjiang 150030,China;2.College of Life Science,Inner Mongolia University for the Nationalities,Tongliao,Inner Mongolia 028000,China;3.College of Life and Environmental Sciences,Harbin Normal University,Harbin,Heilongjiang 150080,China);The Clone of ACS2 Gene of Petunia hybrida and the Construction of Its' Antisense Repetion Expression Vectors[J];Northern Horticulture;2009-07
4 WANG Guang-hui (Department of Biology and Food Engineering,Suihua University,Suihua,Heilongjiang 152061);Research Advance on Molecular Biology of Plant's Sod and Its Applications in Plant Resistance Genetic Engineering[J];Northern Horticulture;2011-03
5 Wu Lili,Gao Fuling,Wang Lei,Zheng Wei,Yang Chuanping,Jiang Tingbo((Key Laboratory of Forest Tree Genetic Improvement and Biotechnology(Northeast Forestry University),Ministry of Education,Harbin 150040,P.R.China);Specific Gene Expression in Stem of Populus simonii × P.nigra and Cloning of PsnLAC Gene[J];Journal of Northeast Forestry University;2011-04
6 Du Guohua Zhang Lijun Fan Jinjuan Ruan Yanye Liu Chun Xu Hongmei Biological Science & Technology College,Shenyang Agricultural University,Shenyang,110866;Protein Specific Degradation Systems in Higher Plants[J];Molecular Plant Breeding;2010-03
7 Li Yuting 1 Lu Zhenhua 1 Niu Liang 1 Han Shiming 2 Wang Zhiqiang 1* 1 Zhengzhou Fruit Research Institute,The Chinese Academy of Agricultural Sciences,Zhengzhou,450009;2 The Chinese Academy of Sciences,Lushan Botanical Garden,Lushan,Jiangxi Province,332900;Preliminary Investigation of Differentially Expressed Genes Using cDNA-AFLP Approach During Growth Ramping of Semi-dwarf Peach[J];Molecular Plant Breeding;2011-01
8 SONG Xin1,2,3,YANG Wenpeng2,3,ZHAO Degang1(1.The Key Lab.of Agro-Bioengineering,Guizhou University,Guiyang,Guizhou 550025;2.Guizhou Institute of Upland Crops, Guiyang,Guizhou 550006;3.Guizhou Key Lab.of Agricultural Biotechnology,Guiyang,Guizhou 550006,China);The cDNA-AFLP Technique and Its Application to the Studies on Plant Genetics and Breeding[J];Guizhou Agricultural Sciences;2007-05
9 QIAO Yan-chun1,LV De-guo1,GUO Dong-liang2,YANG Yun-ying3(1College of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110161 China;2College of life science,South China Normal University,Guangzhou,Guangdong 510631 China;3College of Horticulture,South China Agricultural University,Guangzhou,Guangdong 510642 China);Comparison of methods used for RNA extraction from different tissues of hawthorn fruit and establishment of cDNA-SRAP system[J];Journal of Fruit Science;2009-06
10 WANG Nian,ZHANG Kai-chun*,WANG Jing,ZHANG Xiao-ming,YAN Guo-hua(Institute of Forestry and Pomology,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100093 China);Preliminary study on polymorphism of ACO gene in six varieties of sweet cherry(Prunus avium)[J];Journal of Fruit Science;2012-03
China Proceedings of conference Full-text Database 1 Hits
1 HUANG He~1 WANG Lin-lin~1 WANG Shun-li~(1,2) CAO Hua-wen~1 DAI Si-lan~1 (1 College of Landscape Architecture,Beijing Forestry University,National Engineering Research Center for Floriculture, Beijing 100083;2 Vacation and Technology College;Beijing Agricultural College;Beijing 102206);A Quick and High Efficient Method for RNA Isolation from Chrysanthemum lavandulifolium Applied for cDNA-AFLP[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Di-qiu et al(College of Life Science and Technology,Kunming University of Technology,Kunming,Yunnan 650224);Structural Characteristics of Aquaporins in Plants and Their Expression and Regulation[J];Journal of Anhui Agricultural Sciences;2008-27
2 WANG Guo-qiang(Xian University of Arts and Science,Xian,Shaanxi 710065);Research Progress in Biology Function of Small Heat Shock Proteins[J];Journal of Anhui Agricultural Sciences;2010-19
3 Zhu Qingsong; Liu Zhonghua; Chen Xuemei; Jiang Xiangning; Li Ning. Journal of Beijing Forestry University (2001)23(1)15~17[Ch,16?ref.]College of Plant Sciences, Beijing For. Univ.,100083,P.R.China.;Over expression of high\|plant ACC synthase in Escherichia coli and activity assay of expressed protein.[J];Journal of Beijing Forestry University;2001-01
4 MA Hong-shuang1,2;XIA Xin-li1;YIN Wei-lun1.1 National Engineering Laboratory for Forest Tree Breeding,Beijing Forestry University,100083,P.R.China;2 Preparation Office of Taishan Botanical Garden,Taiyuan,030006,P.R.China.;Cloning and analysis of SCL7 gene from Populus euphratica[J];Journal of Beijing Forestry University;2011-01
5 MA Jun-lian~1,TANG Xia~1,LIU Yue-ying~2,LIU Kai~1,ZHANG Zi-de~1(1.College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, China;2.Biological Science and Engineering College, Hebei University of Ecnomic and Business, Shijiazhuang 050061, China);Cloning of ACC sythase from Mopan and Fuyu persimmon fruits[J];Journal of Agricultural University of Hebei;2004-04
6 JIANG Teng,LIN Yong-xiang,LIU Xue,JIANG Hai-yang,ZHU Su-wen (Anhui Province Key laboratory of Crop Biology,School of Life Science,Anhui Agricultural University,Hefei 230036,China);Genome-wide analysis of the WRKY transcription factor family in Medicago truncatula[J];Acta Prataculturae Sinica;2011-03
7 RAO Jing-ping1,TONG Bin1,Ryohei Nakano2,Akitsugu Inaha2 (1 College of Horticulture,Northwest Science and Technology University of Agriculture and Forestry,Yangling,Shaanxi 712100;2 Faculty of Agriculture Okayama University,Okayama,700-8530,Japan);Cloning and sequence analysis of cDNA encoding for ACC synthase from persimmon fruit[J];Acta Botanica Boreali-occidentalia Sinica;2001-05
8 LIU Ya-li,WANG Xi-ping,ZHAO Ming-de(Department of Horticulture,Northwest Sci-Tech Univeristy of Agriculture and Forestry,Yangling,Shaanxi 712100,China);The latest development about regulation and controlling of cut flowers transportation[J];Acta Botanica Boreali-occidentalia Sinica;2002-01
9 LIU Xian-yan,LIU Ya-li,WANG Yue-jin,ZHANG Zong-qin(Key Laboratory of Northwest Horticulture Plant Germplasm and Genetic Improvement of the Ministry of Agriculture of China,Key Laboratory for Molecular Biology of Agriculture of Shaanxi Province,College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China);Cloning and Sequencing Full-length cDNA Encoding ACC Oxidase in Lilium[J];Acta Botanica Boreali-Occidentalia Sinica;2008-04
10 Liu Yu Shanghai Institute of Plant Physiology, Academia Sinica;BIOSYNTHESIS AND ROLE OF ETHYLENE IN FRUITS DURING RIPENING[J];Discovery of Nature;1986-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved