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《China Biotechnology》 2015-01
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Analysis of ITS Sequences and Identification of Germplasm Resources about Three Cultivars of Pinellia from Jingzhou,Hubei Province

ZHANG Wen-tao;YU Jing;LIU Yi-liang;ZHANG Ben-hou;CHEN Ji-shuang;College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University;Institute of Marine Industry,Nanjing Tech University;  
The genomic DNA were extracted from leaf tissue collected three cultivars of Pinellia,different phenotypes,collected from the field samples in Jingzhou,Hubei Province,which its corresponding the internal transcribed spacer( ITS) sequences were obtained using Polymerase Chain Reaction( PCR) directly and sequenced. The sequence alignment and phylogenetic tree analysis based on NCBI Gen Bank database,combined with the morphological characteristics of plants,indicated that HBB-01 belonged to Pinellia ternata,which is the"Regulations"from China Pharmacopoeia of Pinellia herbs,and HBD-02 belonged to P. tripartita,which is adulterants medicinal of Pinellia ternata. Notably,the result of HBF-03 is complex that its ITS sequence homology is up to more than 80%,which with the Colocasia,Arisaema,Pinellia. In the evolutionary relationship,they also have some connection. It suggested that HBF-03 may belong to varieties from Araceae. different genera but close kinship plants. The deduction needs further argument. In this paper,through the analysis of germplasm origins and phylogenetic relationships about the three cultivars of Pinellia,identificated the main varieties,which is artificial cultivation in Jingzhou,Hubei Province. The result has guided significance for the local medicinal,who have planting to meet "Chinese Pharmacopoeia"specification of P. Ternata. In addition,it has provided a reference basis for protection and breeding of high quality Pinellia resources.
【Fund】: 江苏省科技支撑计划(BE2014408);; 江苏省自然科学基金(BK20141265)资助项目
【CateGory Index】: S567.239
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