Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《China Biotechnology》 2015-01
Add to Favorite Get Latest Update

c-Kit~+ Combining with DiI Micro-label to Sort the Cardiac Telocytes

XIAO Hu-song;SHEN Xiao-tao;ZHENG Xin;LI Yan-mei;CAI Dong-qing;Key Laboratory for Regenerative Medicine,Ministry of Education,Jinan University;The International Base of Collaboration for Science & Technology,The Ministry of Science and Technology & Guangdong Province,Jinan University;Department of Developmental and Regenerative Biology,Ji Nan University;  
Objective: To establish methodology for specific sorting of cardiac telocytes using c-Kit+Combining with Di I Micro-label and flow cytometry. Methods: The cardiac telocytes were isolated via a c-Kit antibody conjugated magnetic bead method. The cells which have unique telocyte morphology, with long"Telopode",were labeled with Di I microinjection. The labeling Di I+cells were sorted and recovered in 96-well plate by single cell flow cytometry technique. The immunofluorescence and RT-PCR were applied to confirm the phenotype of collected cells. Results: The cardiac telocytes( c-Kit+) which have unique telocyte morphology were able to be labeled by Di I microinjection. The Di I labeled cells were able to be sorted and recovered in single cell level. The adherent cells of collected Di I+cells were 14. 9% under culture. The 5. 6% of cultured Di I+cells was able to proliferate and the 2. 4% of cultured Di I+cells was able to form cell-clone. In addition,the formed cell-clone was able to subculture. The immunofluorescence staining confirmed that the recovered Di I+cells were c-Kit-and CD34-positive. RT-PCR results also showed that the recovered Di I+cells were positive for cKit,CD34,Vimentin and PDGFR-β,which are the markers for telocytes. Conclusion: The methodology which was established in present study was able to isolate and sort cardiac telocytes in single cell level. The sorted cardiac telocytes were able to proliferate and maintain the unique phenotype.
【Fund】: 科技部港澳台合作专项(2012DFH30060);; 国家自然科学基金(81170324 81470433);; 广东省教育厅国际合作平台项目(2012gjhz0003);; 广东省自然科学基金重点项目(S2012020010895);; 广东省科技厅国际合作平台项目(2013B051000062)资助项目
【CateGory Index】: R329.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Liu Juan-juan1,2,3,Shen Xiao-tao1,2,3,4,Zheng Xin1,2,3,Li Zhen1,2,3,Wang Jian1,2,3,Qi Xu-feng1,2,3,Cai Dong-qing1,2,3 1Key Laboratory for Regenerative Medicine,Ministry of Education,2Joint Laboratory for Regenerative Medicine,the Chinese University of Hong Kong-Jinan University,3the International Base of Collaboration for Science & Technology,the Ministry of Science and Technology & Guangdong Province Science and Technology Bureau,4Department of Biomedical Engineering,Jinan University,Guangzhou 510632,Guangdong Province,China;Distribution of Telocytes in the rat heart[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2011-19
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 ZHOU Jin;WANG Yan;ZHU Ping;SUN HongYu;MOU YongChao;DUAN CuiMi;YAO AnNing;LV ShuangHong;WANG ChangYong;Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center,Academy of Military Medical Sciences;The First Department of Cardiology, South Building, The General Hospital of People's Liberation Army;Laboratory of Oncology, Affiliated Hospital of Academy of Military Medical Sciences;;Distribution and characteristics of telocytes as nurse cells in the architectural organization of engineered heart tissues[J];中国科学:生命科学(英文版);2014-02
2 XIAO Jing;YU Xi-yong;Medical Research Center of Guangdong General Hospital,Guangdong Academy of Medical Sciences;School of Pharmaceutical Sciences,Southern Medical University;;Research progress on exosomes in cardiovascular diseases[J];Chinese Journal of Pathophysiology;2014-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved