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《China Biotechnology》 2015-01
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Targeted Delivery of siRNA Mediated by Aptamer Modified Liposome

TANG De-ping;MAO Ai-hong;WANG Fang;ZHANG Hong;WANG Li;LIAO Shi-qi;The School of Chemical & Biological Engineering,Lanzhou Jiaotong University;Institute of Gansu Medical Science Research;  
To investigate the efficiency of siRNA using aptamer-conjugated liposome( Apt-LP),A10-3. 2was used as a PSMA-targeting ligand in the design of a liposome-based siRNA delivery system to prostate cancer cells. The sequence of the aptamer used in this study is modified with 3'- Cholesterol and with 2'- Fluor pyrimidines. The cholesterol tag immobilizes the aptamer on the liposome surface by inserting into the hydrophobic lipid membrane. Liposome was conjugated to aptamer and used as a vehicle for siRNA target delivery. GFP expression assays of p EGFP- N1 against LNCa P( PSMA+) and PC-3( PSMA-) cells demonstrated that the transfection efficiency of the synthesized Apt-LP complex was higher than that of the liposome. In addition,gene knock down assay of Apt-LP-siRNA complex in LNCa P( PSMA+) and PC-3( PSMA-) cells showed that Bcl2 gene silencing effect significantly higher than that of LP-siRNA complex and more effectively induced apoptosis of LNCa P cells. These results suggest that the Apt- LP is a kind of effective siRNA targeting delivery system,has potential clinical application.
【Fund】: 甘肃省中青年基金(1107RJYA033);; 兰州交通大学校青年基金(2013012)资助项目
【CateGory Index】: R943
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