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《China Biotechnology》 2015-01
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The Construction and Application of EchDA Genetic Engineering Bacteria

ZHAO Guo-ling;TAO Xin-yi;WANG Feng-qing;WEI Dong-zhi;State Key Laboratory of Bioreactor Engineering,Newworld Institute of Biotechnology,East China University of Science and Technology;  
The EchDA was systematically overexpressed by genetic engineering in the heterologous hosts Streptomyces lividans TK24. Mature peptide coding fragement of Ech DA was amplified from Actinoplanesutahensis NRRL 12052. The expression plasmids p NW-S11,p NW-S12 and p NW-S13 are constructed by inserting Ech DA,Perm E*promoter and PSau3 Apromoter into vector p NW-S1. As a result,The Ech DA was secretion expressed after induction with IPTGand the enzyme activity can reach 125 U / L under the best conditions for industrialized production. With optimization of the reaction conditions with 15 g / Liter FR901379,the molar conversion can achieve 100% within 24 hours. The highest enzyme activity by 300 L fermentation tank can reach 80 U / L,and the molar conversion can achieve100% with 10 g / L FR901379. Ech DA in industry have been relying on fermentationof original producer,Actinoplanesutahensis,which have low yield and activity. The construction of recombinant Ech DA producing strain will be must to improve the current production process of echinocandin antifungal drugs.
【Fund】: 国家“863”科技重大专项资助项目(2012ZX09304009)
【CateGory Index】: R915
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【Citations】
Chinese Journal Full-text Database 2 Hits
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【Co-citations】
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1 OUYANG Hao-miao;JIN Cheng;State Key Laboratory of Mycology,Institute of Microbiology,Chinese Academy of Sciences;;Cell Wall and GPI Structure in Aspergillus fumigatus[J];Guangxi Sciences;2014-02
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5 ZHANG Ying;TAO Xin-yi;WANG Feng-qing;WEI Dong-zhi;National Engineering Research Center for Biotechnology,Newworld Institute of Biotechnology, East China University of Science and Technology;;Promotion Efficiency Comparison of Constitutive Promoters PermE* and PSau3A in Streptomyces Lividans[J];Chemistry & Bioengineering;2013-11
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