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《China Biotechnology》 2015-05
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S100A9 Promotes Human Hepatocellular Carcinoma Cell HepG2 Proliferation and Invasion Involving RAGE

WU Rui;ZHOU Lan;CUI Fang;The First Affiliated Hospital of Chongqing Medical University;Chongqing Medical University;  
Objective: To investigate the biological effect of S100A9 on human hepatocellular carcinoma cell line HepG2 and the relevent mechanism. Methods: Immunohistochemiy and Western blot assay were used to detect S100A9 expression in human hepatocellular carcinoma( HCC) intratumoral and peritumoral tissues.Prokaryotic expression system was used to prepare recombinant protein GST-S100A9. HCC cells HepG2 and live normal cells L02 were treated with GST-S100A9,then MTT assay was used to study the cell proliferation,and invasion assay was used to study cell invasiveness. Western blot assay was used to detect advanced glycation end products( RAGE) expression in HepG2 cells and L02 cells. Results: The expression of S100A9 was higher in HCC intratumoral tissues than that in peritumoral tissues. GST-S100A9 promoted the proliferation and invasion of HCC cells HepG2,but no effect on live normal cells L02. The expression of the receptor for RAGE in HepG2 cells was higher than that in L02 cells. Treatment with RAGE blocking antibody abrogated the S100A9-promoted proliferation and invasion of HepG2 cells,demonstrating that these biological effects were involved in RAGE.Conclusion: S100A9 promotes the proliferation and invasion of HepG2 cells involving RAGE.
【Fund】: 国家临床重点专科建设项目(20100305);; 国家自然科学基金(30772548)资助项目
【CateGory Index】: R735.7
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