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《China Biotechnology》 2015-05
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Over Expression of Arabidopsis CAT Improved the Absorption and Tolerance of Gas HCHO in Tobacco

HAN Shuang;YANG Zhi-li;CHEN Li-mei;Biotechnology Research Center,College of Life Science and Technology,Kunming University of Science and Technology;  
To improve the absorption and tolerance of HCHO in plants,the plant expression vector pk2-Prbc S-*T-CAT was constructed using Arabidopsis thaliana CAT( catalase) coding region. Overexpression of CAT in wild-type( WT) tobacco chloroplasts produced two transgenic lines( C1,C3) which had different expression quantity. Using 10 ppm,20ppm and 40 ppm gas HCHO treated WT and transgenic tobacco,and then analyzed the HCHO absorption efficiency and tolerance of CAT transgenic tobacco. The results showed that the absorption quantity of gas HCHO by transgenic tobacco was higher than wild type( WT), and with the increasing concentration,total soluble glucide,total protein and total chlorophyll content in leaves of the transgenic lines were significantly higher than WT. The oxidative damage index of H2O2,malondialdehyde(MDA) and protein carbonyl( PC) which content in transgenic lines leaves were significantly lower than the WT. The PM H+-ATPase and hydrogen pump activity of transgenic tobacco( C1) was 3 times and 2. 5 times than WT after 40 ppm HCHO treatment. Stomatal conductance of C1 is 2. 67 times than WT. These results indicated that overexpression of Arabidopsis CAT could reduce oxidative damage in tobacco cells which induced by HCHO,and improved the ability of tobacco to HCHO detoxification and enhanced the absorption and resistance of tobacco to HCHO. It was revealed that the stomatal conductance affected by PM H+-ATPase and hydrogen pump activity when gas HCHO stressed tobacco,which might be one of the causes of higher HCHO absorption efficiency of transgenic tobacco.
【Fund】: 国家自然科学基金资助项目(30970263)
【CateGory Index】: X173
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