Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《China Biotechnology》 2015-05
Add to Favorite Get Latest Update

Over Expression of Arabidopsis CAT Improved the Absorption and Tolerance of Gas HCHO in Tobacco

HAN Shuang;YANG Zhi-li;CHEN Li-mei;Biotechnology Research Center,College of Life Science and Technology,Kunming University of Science and Technology;  
To improve the absorption and tolerance of HCHO in plants,the plant expression vector pk2-Prbc S-*T-CAT was constructed using Arabidopsis thaliana CAT( catalase) coding region. Overexpression of CAT in wild-type( WT) tobacco chloroplasts produced two transgenic lines( C1,C3) which had different expression quantity. Using 10 ppm,20ppm and 40 ppm gas HCHO treated WT and transgenic tobacco,and then analyzed the HCHO absorption efficiency and tolerance of CAT transgenic tobacco. The results showed that the absorption quantity of gas HCHO by transgenic tobacco was higher than wild type( WT), and with the increasing concentration,total soluble glucide,total protein and total chlorophyll content in leaves of the transgenic lines were significantly higher than WT. The oxidative damage index of H2O2,malondialdehyde(MDA) and protein carbonyl( PC) which content in transgenic lines leaves were significantly lower than the WT. The PM H+-ATPase and hydrogen pump activity of transgenic tobacco( C1) was 3 times and 2. 5 times than WT after 40 ppm HCHO treatment. Stomatal conductance of C1 is 2. 67 times than WT. These results indicated that overexpression of Arabidopsis CAT could reduce oxidative damage in tobacco cells which induced by HCHO,and improved the ability of tobacco to HCHO detoxification and enhanced the absorption and resistance of tobacco to HCHO. It was revealed that the stomatal conductance affected by PM H+-ATPase and hydrogen pump activity when gas HCHO stressed tobacco,which might be one of the causes of higher HCHO absorption efficiency of transgenic tobacco.
【Fund】: 国家自然科学基金资助项目(30970263)
【CateGory Index】: X173
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 ZHAO Hui et al (Horticulture College,Jinling Institute of Technology,Nanjing,Jiangsu 210038);Effects of Formaldehyde Pollution on Leaf Protective Enzymatic Activities of 3 Kinds of Indoor Leaf-viewed Plant[J];Journal of Anhui Agricultural Sciences;2009-32
2 ;The Polluting Source of Indoor Air and its Harm[J];Fujian Environment;2001-05
3 CHEN Wenrong,LIU Peng,XU Gendi,YU Hui′na,DONG Li(College of Chemistry and Life Science,Zhejiang Normal University,Jinhua Zhejiang321004,China);Effects of calcium applicated on alleviation of aluminum toxicity to soybean[J];Journal of Zhejiang Normal University(Natural Sciences);2008-02
Chinese Journal Full-text Database 10 Hits
1 LU Chang-gen et al (Institute of Botany of Jiangsu Province and Chinese Academy of Sciences,Nanjing,Jiangsu 210014);Study on Four Kinds of Indoor Ornamental Plants' Responses to Benzene Gas Stress[J];Journal of Anhui Agricultural Sciences;2008-34
2 XU Di et al(Biotechnology Research Center,College of Life Science and Technology,Kunming University of Science and Technology,Kunming,Yunnan 650224);Research on Absorptive Capacity of Ornamental Plant Leaves on Formaldehyde[J];Journal of Anhui Agricultural Sciences;2009-12
3 WU Zhi-quan et al(College of Life Science,South China Agricultural University,Guangzhou,Guangdong 510642);Effects of Calcium on Activity of Antioxidative Enzymes in Rice Seedlings under Aluminum Stress[J];Journal of Anhui Agricultural Sciences;2011-35
4 ZHOU Jia-yu ZHU Meng-jiao ZHANG Lei XU Xiao-gang (College of Forestry Resource and Environment,Naniing Forestry University,Nanjing Jiangsu 210037);Overview of Research on Purification of Indoor Plants[J];Modern Agricultural Sciences and Technology;2010-18
5 Yang Yuanqiang,Guo Zhongling(College of Forestry,Beihua University,Jilin 132013,China);The Positive and Negative Effects of Household Flowers to Indoor Air Pollution[J];Environmental Science and Management;2009-05
6 YAU Yiu-hung,HO Kin-chung,XU Jing-liang,LEUNG Kwan-lung,LEUNG Yu-hin(Environmental Studies & Safety Science,School of Science and Technology, The Open University of Hong Kong,Hong Kong SAR,China);Evaluation of Two House Plants for Indoor Air Purification[J];Urban Environment & Urban Ecology;2011-04
7 HE Dengliang DENG Yuequan DONG Faqin XU Guangliang (Department of Material Science and Engineering,Southwest University of Science and Technology,Mianyang 621010);Building Materials Used to Prevent Radon and Radiation[J];Materials Review;2005-09
8 ZHANG Qiang~1 DENG Yuequan~1 DONG Faqin~1 XU Guangliang~1 YANG Rui~1 HE Dengliang~2 1 Materials Science-and Engineering College of Southwest University of Science and Technology,Mianyang 621010 2 Chemistry Department of Mianyang Normal College,Mianyang 621010;Current Study Situation of Radon Pollution and Prevention of Building Material Based on Industrial Waste Residue[J];Materials Review;2007-10
9 LING HU Yu-wei,LI Bin,LI Si-feng,ZHANG Ying,LIU Li-cheng(Xi'an Botanical Garden of Shaanxi Province,Institute of Botany of Shaanxi Province,Xi'an 710061,China);Monitoring,Purification and Response of Three Indoor Ornamental Plants on Formaldehyde Pollution[J];Acta Botanica Boreali-Occidentalia Sinica;2011-04
10 WU Kong-huan1,CHEN Qi1,LI Kun-zhi1,YU Yong-xiong2,CHEN Li-mei1* (1 Biotechnology Research Center,Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China;2 College of Zoological Science and Technology,Southwest University,Chongqing 400716,China);Effects of Aluminum Stress on Membrane Lipid Peroxidation and Antioxidant Enzyme Activities in Black Soybean[J];Acta Botanica Boreali-Occidentalia Sinica;2012-03
China Proceedings of conference Full-text Database 1 Hits
1 MAO Min-hui ZHANGJIANG Xuan-zi CAO Xiao-yue PAN Hui-tang LV Ying-min (College of Landscape Architecture,Beijing Forestry University,National Engineering Research Center for Floriculture,Beijing 100083);Progress on the Research of Reducing Indoor Benzene Content by Using Ornamental Plants[A];[C];2010
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 MU Li-li(College of Plant Protection Nanjing Agricultural University,Nanjing Jiangsu 210095);Influence of Methanol on Plant Growth[J];Modern Agricultural Sciences and Technology;2010-11
2 WEI Mei-hong1,LI Ji-mi1,MA Hua-zhang1,CHEN Deng-long2,LI Min1(1 College of Life Science, Fujian Normal University, Fuzhou 350007, China; 2 College of Chemistry and Material Science, Fujian Normal University, Fuzhou 350007, China);Response to SOD of Aloe vera var. chinensis (Haw) Berg. by formaldehyde[J];Journal of Safety and Environment;2007-02
3 XU Di,CHEN Li-mei,NIAN Hong-juan,MEI Yan (Biotechnology Research Center,College of Life Science and Technology,Kunming University of Science and Technology,Kunming 650224,China);Research Progresses on One-carbon Compound Metabolism in Higher Plants[J];Acta Botanica Boreali-Occidentalia Sinica;2009-06
4 Xu Difa, Zhong Tianxiang,Xu Dongmei, Liu Guangshen (Institute of Environment, Zhejiang University, Hangzhou 310027);EFFECTS OF ACID DEPOSITION ON SOIL GEOCHEMICAL CYCLES[J];Geology-geochemistry;2002-04
5 WU Kai,YANG Guang-tao,LOU Xiao-hua,PENG Guang-yin,WANG Li-ming,LI Yan,KE Xiang-hong,YANG Xu.College of Life Science,Central China Normal University,Wuhan 430079,China;DNA-protein crosslinks and DNA damages of mice lung induced by formaldehyde[J];Journal of Public Health and Preventive Medicine;2006-02
6 WANG Li-ying,YANG Zhen-de,DENG Rong-yan,QIN Shou-yi(Forestry College,Guangxi University,Nanning,Guangxi,530005,China);Study on Reaction of Several Garden Plans to Formaldehyde Pollution[J];Guangxi Sciences;2007-02
7 DUAN Yun-qing(College of Arts and Sciences,Shanxi Agricultural University,Taigu,Shanxi 030801);Effects of Cd stress on the activity of POD,PPO and SOD of two varieties of pakchoi[J];Guangxi Agricultural Sciences;2006-01
8 ZHENG Ai-zhen1,2,LIU Chuan-ping2,SHEN Zhen-guo2(1.Department of Biology,Shangqiu Teachers College,Shangqiu Henan 476000,China;2.College of Life Sciences, Nanjing Agriculture University, Nanjing 210095,China);Effect of Cadmium on MDA Content, POD and SOD Activities of Brassica Pekinensis and Brassica Chinensis[J];Hubei Agricultural Sciences;2005-01
9 HuangQiaoyun et .al. (Dept. of Soil Science, HuazhongAgricultural University, Wuhan 430070);Alleviating Effect of Silicon on Aluminum Toxicity to Wheat Growth in Acid Soil[J];CHINESE JOURNAL OF ENVIROMENTAL SCIENCE;1995-06
10 YANG Danfeng, XI Zhuge, ZHANG Huashan, LI Guanxian, CHAO Fuhuan (Institute of health and environmental medicine ,academy of military medical sciences,Tianjin 300050);Oxidation damage of formaldehyde inhalation on rat multiple organs[J];Acta Scientiae Circumstantiae;2004-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved