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《China Biotechnology》 2015-05
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Fermentative Production of L-lactic Acid from Wastepaper by Recombinant Escherichia coli WL204

XIONG Yuan-yuan;LU Chuan-dong;TAO Ye;ZHAO Jin-fang;Hubei Cooperative Innovation Center for Industrial Fermentation,Key Laboratory of Fermentation Engineering Ministry of Education,Hubei University of Technology;  
Previous studies,a recombinant E. coli WL204 was constructed,which contained a chromosomal integrated ldh L gene and possesses the capability of homofermentation of L-lactic acid using xylose as substrate.Wastepaper was selected as feedstock to evaluate the fermentative production of lactic acid by recombinant E. coli WL204. The wastepaper chips were treated with 1∶ 6- 1∶ 14( w / v) H2SO4,and then incubated 60 h at 50℃ with cellulase. The resultant hydrolyzates were detoxified with dried Ca( OH)2,which could remove most of furfural and HMF. Fermentation of the detoxified hydrolyzate of wastepaper with E. coli WL204 produced 31 g L-lactic acid from 100 g dry wastepaper and the lactic acid yield coefficient was 79%. These results demonstrated that WL204 has the potential for homofermentative production of L-lactic acid using cellulosic biomass derived substrates.
【Fund】: 湖北自然科学基金资助项目(2011CDB076)
【CateGory Index】: TQ921.3
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