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《China Biotechnology》 2015-05
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The Establishment of the Methodology used for In Situ Detecting Lentiviral Vector Transcriptional Read-through

GAO Yue;TAN Shuo;REN Zhao-rui;ZHANG Jing-zhi;Shanghai Institute of Medical Genetics,Children's Hospital of Shanghai.Children's Hospital Affiliated to Shanghai Jiao Tong University;Collaborative Innovation Center for Biotherapy,West China Hospital,Sichuan University;Key Laboratory of Embryo Molecular Biology,Ministry of Health & Shanghai Key Laboratory of Embryo and Reproduction Engineering;  
As a gene delivery system,Lentiviral vector( LVV) has been being increasingly used in gene therapy trials,and drawn large attention in recent years. Yet,transcriptional read-through( TRT) is one of potential risks in its application. For the purpose of improving the detection methods of TRT,and making the results intuitive and accurate,a codon optimized reporter gene,Lac Z,was placed downstream to the 3'- LTR of the proviral vector to simulate its state in chromosome. Upon the transfection followed with Lac Z staining,the cells with TRT phenomenon would be stained blue,and the TRT rate would therefore be visualized at the translational level. Correlating well with the outcomes of q RT-PCR,the established methodology could reflect the feature of TRT,which paves a way for improving LVV biosafety for clinical applications
【Fund】: 国家自然科学基金资助项目(81271690)
【CateGory Index】: R373;Q78
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Jiaping He1,Yudan Fang1,2,Fan Zhang1,Fengqiang Sun1,Juan Wang1,2,and Jingzhi Zhang1,2 1 Shanghai Institute of Medical Genetics,Children’s Hospital of Shanghai,Children’s Hospital Affiliated to Shanghai Jiao Tong University,Shanghai 200040,China 2 Key Laboratory of Embryo Molecular Biology,Ministry of Health & Shanghai Key Laboratory of Embryo and Reproduction Engineering,Shanghai 200040,China;Establishment of the methodology for quantifying lentiviral vector transcriptional read-through rate[J];Chinese Journal of Biotechnology;2013-07
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 SUN Yong1,TANG Bi-kui1,2,BAI He-lu1,ZHU Nai-shuo1(1.Department of Microbiology and Microbial Engineering,School of Life Sciences,Fudan University, Shanghai 200433,China;2.Department of Life Sciences,Bengbu Medical College,Bengbu 233030,China);Expression of HBV-X Gene Induced by Lentiviral Vector in Mice[J];Journal of Fudan University(Natural Science);2013-04
2 CUI Yu-qian;ZHAO Han;MA Zeng-xiang;ZHAO Shi-dou;XU Hai-jing;WANG Wen -ting;GUO Hai -peng;CHEN Zi -jiang;Reproductive Medical Center,Renji Hospital,Shanghai Jiao Tong University School of Medicine;;Construction of the Recombinant Lentiviral Vector Containing Mouse Tox3 Gene and Its Expression in Primary Granulosa Cells[J];Journal of International Reproductive Health/Family Planning;2014-02
3 Zhang Chan;Dong Wenbin;Zhao Shuai;Li Qingping;Kang Lan;Lei Xiaoping;Guo Lin;Zhai Xuesong;Department of Newborn Medicine,Affiliated Hospital,Luzhou Medical College;;Study on lentiviral vector target inducing p66shc gene silencing[J];Chongqing Medicine;2015-01
4 Liu Jinyu;Han Chuangye;Zhao Chunjie;Chen Shou;Yang Jinsong;Department of Spine Surgery,the First Affiliated Hospital of Guangxi Medical University;;EXPRESSION OF GREEN FLUORESCENT PROTEIN GENE IN HUMAN LIGAMENT FIBROBLASTS MEDIATED BY LENTIVIRAL VECTOR[J];Journal of Guangxi Medical University;2015-01
5 WANG Yuan;ZHANG Qi;ZHANG Yanxia;ZHANG Jingjun;XIA Rong;Department of Blood Transfusion,The Affiliated Huashan Hospital of Fudan University;;Immature dendritic cell modified with SOCS1 gene and its role on prevention of graft-versus-host disease in mouse[J];Chinese Journal of Blood Transfusion;2015-05
6 YANG Dong-chang,MU Xiao-xin,LIU Qiao-yu,DENG Lei,CHEN Yun1*,SUN Bei-cheng*(Department of Liver Surgery,the First Affiliated Hospital of NJMU,Nanjing 210029;1Department of Microbiology and Immunology,NJMU,Nanjing 210029,China);Construction of the recombinant lentiviral vector containing human ANXA2 gene and its expression in hepatocellular carcinoma cells[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2011-02
7 DENG Hai-yan,ZENG Jun-yi,WEI Yun-feng(The First Affiliated Hospital of Nanchang University,Jiangxi 330000,China);Construction and identification of recombinant lentiviral vector encoding miR-1 of rat[J];Laboratory Medicine and Clinic;2013-02
8 Chen Zhi;He Shaohua;Jiang Shuai;Liu Peng;Bai Luyue;Fen Zhengqin;Zhu Jin;Chen Renjie;Department of Otolaryngology,the Second Affiliated Hospital of NJMU;Department of Oncology,the First Affiliated Hospital of NJMU;Huadong Medical Institute of Biotechniques;Key Laboratory of Antibody Technique of Ministry of Health;;Effects of EB virus latent membrane protein 2A on human nasopharyngeal carcinoma cell proliferation and migration[J];Journal of Nanjing Medical University(Natural Sciences);2015-04
9 JIANG Lei,LIN Fei-yan,WANG Fu-le,YE Ai-fang,WU Jian-bo.Laboratory of Internal Medicine,The First Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,China;Construction of lentiviral vector expressing TIEG1 gene with breast cancer specific promoter and identification of activity[J];The Journal of Practical Medicine;2011-11
10 Ji-dong WANG,Shuang LUO,Hong-juan PAN,Zhen-wei YAO (Department of Obstetric and Gynecology of the First Affiliated Hospital of Chongqing Medical University,Chongqing,400010);Transfection of Lentivirus Vectors Mediated Green Fluorescence Protein Gene into Rats' Ovaries In Vivo[J];Reproduction & Contraception;2012-06
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 MA Hai-yan,FANG Yu-dan,ZHANG Jing-zhi(School of Medicine,Shanghai Jiaotong University,Shanghai Children’s Hospital,Shanghai Institute of Medical Genetics,Shanghai 200040,China);A Novel Method for the Determination of Recombinant Lentiviral Titer and Infectivity by qRT-PCR[J];Life Science Research;2009-05
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