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《China Biotechnology》 2015-05
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Construction of an Inducible Expression Shuttle Vector for Ketogulonigenium vulgare Allowing Tightly Regulable Gene Expression

LUO Wan-yue;LI Tian-ming;YU Ying;XU Mei-xue;YI Hong;Hebei University of Science and Technology;  
The genes encoding tet repressor proteins and Ketogulonigenium vulgare sorbose dehydrogenase operator which inserted operon teto were synthesized by overlapping PCR. The shuttle vector of tetracycline induced expression with broad host plasmid p BBR1MCS-5 was constructed and transformed Ketogulonigenium vulgare. The resistance of kanamycin was regulatively expressed after getting the positive recombinant strains.The results show that the cultivation of recombinant strains could grow in the medium containing kanamycin where added 0. 4mg / ml tetracycline inducers after 2 hours,while they can't grow in the medium containing kanamycin where didn 't add tetracycline inducers. The optimal induction of tetracycline concentration determined was0. 6mg / ml.
【CateGory Index】: Q78;Q93
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