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《China Biotechnology》 2015-05
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The Process of Methods on Validating the Differential Transcription of Genes

ZUO Hai-yang;CHEN Xiao-li;CAI Yong;HAO Hai-sheng;QIN Tong;ZHAO Xue-ming;LU Yong-qiang;WANG Dong;Animal Science and Technology College,Jilin Agricultural University;The Key Laboratory for Farm Animal Genetic and Utilization of Ministry of Agriculture of China,Institute of Animal Science,Chinese Academy of Agricultural Science;Animal Husbandry Station of Beijing;  
The differential expression of genes is closely related to biological characters and function of tissue and cell. With the extensive study on the differentiation of tissue and organs and the growth and development of cells and individuals,the validation of differentially expressed genes are getting more and more important. Thus,a series of validating techniques of differentially expressed analysis were established on the basis of nucleic acid hybridization and PCR, such as Northern blot and fluorescence quantitative PCR. The differentially expressed genes were validated of different treatment,different organs and different development period using these methods,which laid a solid foundation for the gene functional analysis. With the fast development of the detecting methods,the validation methods of differential expression of genes has developed from qualitative to quantitative,from tedious and complicated to simple and rapid,from requiring a large number of initial RNA to only a small amount of RNA,even the fluoresce quantitative PCR method for single cell has been established,and the method is to be more efficient,accurate and low cost. However,The validation for the samples difficult sampling like germ cell and less amount of RNA containing like sperm is still a great challenge for the current method. The validation methods of differential expression of genes from the perspective of the development process was reviewed. Reference to the detedtion of samples dificult sampling and less amount RNA containing was provided.
【Fund】: 国家自然科学基金(31372296);; 奶牛产业技术体系北京市创新新团队项目:中国农业科学院家畜胚胎工程与繁殖创新团队项目(ASTIP;cxgc-ias-06)资助项目
【CateGory Index】: Q78
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