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《China Biotechnology》 2015-11
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Preparation and Characterization Testing of Bone Morphogenetic Protein2/Pearl Powder/Chitosan Porous Scaffolds

LI Ting;SUN Jing;ZHAO Xiang-zhe;LIAN Li-qiang;XIE Fu-qiang;School of Stomatology,Lanzhou University;The Second Hospital of Lanzhou University;  
Objective:To prepare the bone morphogenetic protein-2/pearl powder/chitosan composite scaffolds and observe biological properties.Methods:The bone morphogenetic protein-2/pearl powder/chitosan porous scaffolds were prepared by freeze-drying.The surface properties,the porosity,and the thermal stability of the stent were observed by light microscopy and scanning electron microscopy(SEM),pycnometer,and TGA respectively.Then the rabbit bone marrow mesenchymal stem cells were co-cultured with the stent to detect cell adhesion properties,and inflammatory reaction was observed by burying the stent in subcuticle of rats.Results and conclusion:The results showed that the pore size of bone morphogenetic protein 2/pearl powder/chitosan scaffold ranged between 100μm and 300μm with porosity being 91.64% and compressive stresses up to3.37 MPa.The stent,had suitable cell adhesion properties,and the histocompatibility is predominant,suggesting that the stent may be used as Bone Tissue Engineering materials applied for clinical repairment for bone tissue defects.
【Fund】: 甘肃省自然科学基金资助项目(145RJZA149)
【CateGory Index】: R783.1
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