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《China Biotechnology》 2015-11
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The Effects of ste3 and ste4 Genes Double Disruption in Ebosin Biosynthesis

BAI Li-ping;JIANG Rong;GUO Lian-hong;ZHANG Yang;LI Yuan;Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences & Peking Union Medical College;  
Objective:The biosynthesis cluster(ste) of a novel exololysaccharide called Ebosin producing by Streptomyces had been identified previously,that ste3 and ste4 maybe encode a membrane spanning protein probably for sugar import on the basis of bioinformatics analysis.This work studied If ste3 and ste4 were involved in Ebosin biosynthesis.Methods:the ste3 and ste4 genes were disrupted together with a double crossover via homologous recombination.The mutant strain was identified by southern blot and gene complementation also performed.The production of Ebosin through isolating it from wild type,mutant and complementation was analyzed.Results:Ebosin isolated from the supernatants of fermentation cultures of the mutant strain was 153 mg/L,remarkably lower than that of the wild-type strain(319 mg/L).Complementation of the mutant strain resulted in a recovery of the EPS production(299 mg/L) comparing with the wild-type strain.Conclusion:This research firstly elucidate that genes ste3 and ste4 encoding a membrane spanning protein involved in Ebosin biosynthesis and lay the foundation for studying the relationship between primary and second metabolism of Streptomyces sp.139.
【Fund】: 国家自然科学基金(31070086);; 北京市自然科学基金(5092020);; 北京协和医学院协和青年科研基金(3332014009;3332015166);; 中央级公益性科研院所基本科研业务专项(IMBF2015011)资助项目
【CateGory Index】: TQ464.1;Q78
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