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《China Biotechnology》 2015-11
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Effects of Glucose on Cell Growth and Lipid Synthesis of Monoraphidium sp.FXY-10 Under Mixotrophic and Heterotrophic Cultivations

CHE Rao-qiong;HUANG Li;WANG Lin;ZHAO Peng;LI Tao;YU Xu-ya;Faculty of Life Science and Technology,Kunming University of Science and Technology;  
The effects of initial glucose concentrations on cell growth of Monoraphidium sp.FXY-10 under mixotrophic and heterotrophic cultivations were investigated by Andrew equation.A kinetic model was developed to describe the dynamics of glucose consumption for FXY-10 growth.Meanwhile,the influence of different glucose concentrations on the production of lipid by FXY-10 was studied.And the kinetic models of FXY-10 lipid synthesis in the different trophic types with the most appropriate glucose concentration were also constructed.The results showed thatwhen the concentration of glucose was 10 g/L,the maximum total lipid content and biomass concentration were achieved under mixotrophic and heterotrophic conditions.The semi-saturation constants of Monoraphidium sp.FXY-10 to glucose in the mixotrophic(KSJ) and heterotrophic(KSY) conditions were 1.89 g/L and 17.82 g/L,respectively.It implied that the glucose absorption capacity of FXY-10 in the mixotrophic condition was relatively higher than that in the heterotrophic.
【Fund】: 国家自然科学基金资助项目(21266013)
【CateGory Index】: Q949.2
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