Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《China Biotechnology》 2015-11
Add to Favorite Get Latest Update

A Novel Aptasensor for Electrochemistry Detection of β-agonists

YANG Min;CHEN Dan;YAO Dong-sheng;XIE Chun-fang;LIU Da-ling;College of Life Science and Technology,Jinan University;Institute of Microbial Technology,Jinan University;National Engineering Research Center of Genetic Medicine,Jinan University;  
β-agonists are phenylethanolamines with different substituent groups on the aromatic ring and the terminal amino group which have the effect of nutrition redistribution and could accumulate in body tissues causing acute or chronic poisoning when consumed.Therefore,establishing a method for rapid screening of β-agonists is very important to food safety control.The aptamer named AP-Ago is screened by Isothermal Titration Calorimetric method.AP-Ago is a single-strand DNA and 22 base-pairs.The dissociation constant(Kd) to PHL is 3.34 × 10-5mol/L.A labeled free electrochemical aptasensor is developed with AP-Ago,which is sensitive to phenylethanolamine(PHL),clenbuterol(CLB),ractopamine(RAC),salbutamol(SAL) and procaterol(PRO).The detection limits are 0.04 ng/ml(RAC),0.35 pg/ml(CLB),1.0pg/ml(PHL),0.53 pg/ml(SAL) and 1.73 pg/ml(PRO),respectively.The detection time is 15 min.This aptasensor also have a good reproductivity of the relative standard deviation(RSD) at 2.09%.The aptasensor can be developed as a rapid screening means with β-agonists(may be one or more) in sample.
【Fund】: 广东省农业领域科技计划重点项目(2012A020200003);; 暨南大学科研培育与创新基金研究项目(21612453)资助项目
【CateGory Index】: S859.84
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 2 Hits
1 CHEN Dan;YAO Dong-sheng;XIE Chun-fang;LIU Da-ling;Institute of Microbial Technology;National Engineering Research Center of Genetic Medicine;Biological Engineering,Jinan University;;Development of an Aptasensor for Electrochemical Detection of Tetracycline[J];China Biotechnology;2013-11
2 ZHOU Ni;CHEN Dan;YAO Dong-sheng;XIE Chun-fang;LIU Da-ling;Institute of Microbial Technology,Jinan University;National Engineering Research Center of Genetic Medicine;Department of Bio-engineering,Jinan University;;Development of An Electrochemical Aptasensor Basic on the ssDNA Aptamer of Ractopamine[J];China Biotechnology;2014-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 TAN Wei ZHANG Yi~a HONG Ai-Hua LI Huan-Yong WU Wei-Dong~a HUA Hong-Hui~a (Analytical and Testing Center,Jinan University,Guangzhou 510632,P.R.China) a(Food Inspection and Quarantine Technology Center of Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen,Guangdong 510845,P.R.China);Simultaneous Determination of Seven β_2-Receptor Stimulants in Pork by Liquid Chromatography-Tandem Mass Spectrometry[J];Chinese Journal of Spectroscopy Laboratory;2013-02
2 Qiu Qidong;Li Cong;Qi yan;Li Minghao;Cui Haiping;Li Jinqing;Guangdong Testing Institute of Product Quality Supervision;;Determination of Clenbuterol in Lean pork by UPLC-MS/MS[J];Guangdong Chemical Industry;2014-10
3 WANG Wen-jun;LI Yang;YE Yun-feng;JIANG Hai-yang;Beijing WDWK Biotechnology Co.,Ltd.;College of Veterinary Medicine,China Agricultural University;Guangxi Institute of Veterinary Drug Control;;Research Progress on Analytical Methods of Ractopamine in Biological Matrices[J];China Animal Husbandry & Veterinary Medicine;2015-01
4 SHAO Xin-tian;TAO Li-ying;YUE Jing-li;College of Pharmacy,Liaoning Medical University;Department of Chemistry,Jinzhou Teacher's Training College;;Preparation and application of coated carbon clorprenaline hydrochloride selective electrode modified with Fe_3O_4 nanoparticles[J];Journal of Molecular Science;2015-05
5 HUANG Lu-min;YANG Wei-cheng;LUO Yong;HAN Sheng;YANG Chao;XIE Long;LI Mei-hua;SUN Wen;PAN Jie;Shanghai Institute Of Technology;Shanghai Research Institute of Chemical Industry;National Isotope Engineering Technology Research Center-Shanghai Sub-Center;;Synthesis of Tulobuterol-D_9 Labeled by Stable Isotope[J];Fine Chemicals;2015-05
6 LU Jun-li;GE Wei-dong;SUN Jian-xia;YANG Yong;JIAO Rui;OU Shi-yi;BAI Wei-bin;Department of Food Science and Engineering,Ji'nan University;College of Food Science,Sichuan Agricultural University;Yili Maternal and Child Health Care Hospital;Faculty of Chemical Engineering and Light Industry,Guangdong University of Technology;;Research Progress of Aptamer in Detecting Harmful Substances in Food[J];Journal of Agricultural Science and Technology;2015-02
7 WEN Yi;YAO Ruihua;SUN Hongliang;WANG Qiang;ZHANG Wenjing;Chinese Academy for Environmental Planning;;Simultaneous determination of 23 drug residues in groundwater using UPLCMS/MS[J];Acta Scientiae Circumstantiae;2015-07
8 HUANG Yan-mei1 HUANG Zhuo-huan2 FENG Rong-hua1 LI Dong-xia2 LAI Wei-hua1 (1.State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang,Jiangxi 330047,China; 2.Jiangxi Agricultural Product Quality Safety and Inspection Center,Nanchang,Jiangxi 330077,China);Detection of 9 β-agonists multiresidue in pig urine by liquid chromatography-mass spectrometry[J];Food & Machinery;2013-01
9 PENG Tao1 LAI Wei-hua1 ZHANG Fu-sheng2 WANG Qian2 YE Qin2 ZHANG Li2 ZHANG Zhi-fang2 (1.State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang,Jiangxi 330047,China;2.Jiangxi Agricultural Product Quality Safety and Inspection Center,Nanchang,Jiangxi 330077,China);Research progress of properties and detection methods of 20 kinds of β_2-agonist[J];Food & Machinery;2013-03
10 MA Li-li;LIU Yan;JIA Li;FENG Yue-chao;ZHANG Chun-mei;FAN Xiao-jing;Beijing Center for Physical and Chemical Analysis,Beijing Engineering Research Center of Food Safety Analysis;;Solid Phase Extraction Coupled with Ultra Performance Liquid Chromatography-Mass Spectrometry for the Determination of 7 β_2-agonists in Milk[J];Food Research and Development;2015-12
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved