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《China Biotechnology》 2015-11
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The Advance and Application of CRISPR/Cas9 Mediated Genome Editing Technique

PU Qiang;LUO Jia;SHEN Lin-yuan;LI Qiang;ZHANG Yi;ZHANG Shun-hua;ZHU Li;College of Animal Science and Technology,Sichuan Agricultural University;Sichuan Province General Station of Animal Husbandry;Department of Animal Science,Xichang College;  
Along with the dramatic advances in sequencing technology,whole genome sequences of more and more species have obtained.Faced with such a status,site-directed genome editing technique has been adapted as an efficient gene-targeting technology to obtain the gene function and application information.CRISPR/Cas9 is the most effective editing technique by far.CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISPR-associated) refers to an adaptive immune system that is gained from the long-term evolution of the organism which is widespread in bacteria and archaea.The characteristics and development,as well as the application prospect of the technique are summarizzed.
【Fund】: 四川省科技支撑计划(2013NZ0041);; 国家科技支撑计划(2013BAD20B07);; 四川省科技富民强县专项行动计划资助项目
【CateGory Index】: Q789
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【Citations】
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【Co-citations】
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1 ZHU Xiangying;WANG Qingliang;WU Qing;SHEN Qiang;JI Guoqing;Shanghai Genechem Co. ,Ltd;;In vivo Donor Linearization by Ⅰ-SceⅠ can Increase Talen-mediated-knock-in Efficiency[J];Jiangxi Science;2015-03
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3 Liu Jing;Pan Qiuzhen;Zhao Wenbo;Wang Jingyi;Zong Yan;Lian Zhengxing;College of Life Science and Technology,Heilongjiang Bayi Agricultural University;College of Animal Science and Technology,China Agricultural University;;Targeting Rabbit Myostatin Gene through CRISPR/Cas 9 Technology[J];Journal of Heilongjiang Bayi Agricultural University;2015-06
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5 WANG Meng-Yao;YANG Yi-Nong;BIAN Hong-Wu;College of Life Sciences,Zhejiang University;)Huck Institutes of Life Sciences,Pennsylvania State University;;New Genome Targeting Modification Technology Using a CRISPR-Cas System[J];Chinese Journal of Biochemistry and Molecular Biology;2014-05
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7 ZHANG Min-Jing;ZHANG Chun-Hong;SUN Xue-Peng;LI Hong-Ye;ZHU Zeng-Rong;Institute of Entomology Science, Zhejiang University;Institute of Biotechnology, Zhejiang University;;CRISPR/Cas: the emerging tool for insect genomic study[J];Chinese Bulletin of Life Sciences;2015-01
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9 ZHI Da-Long;NIU Yu-Yu;JI Wei-Zhi;Faculty of Life Science and Technology,Kunming University of Science and Technology;Yunnan Key Laboratory of Primate Biomedical Research;;Genome Editing Technologies Based on Engineered Nuclease[J];Chinese Journal of Biochemistry and Molecular Biology;2015-11
10 Andrew R.Bassett;Ji-Long Liu;MRC Functional Genomics Unit, University of Oxford, Department of Physiology, Anatomy and Genetics;;CRISPR/Cas9 and Genome Editing in Drosophila[J];遗传学报(英文版);2014-01
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