Studies of Protective Properties of PEG and Dextran on Hybridoma Cell Culture
Xu Dianshen;Chen Guohao; Yu Juntang; Lu Bing(Dep. of Biochemical Engineering, ECUCT, Shanghai 200237)
Using 2F7 murine hybridoma cell which secrete monoclonal antibody against small lung cancer,the potential toxicity and restricting adding concentration of different protective agents such aspolyethylene glycol (PEG)and dextran were studied. The protective effects at high agitation speed andhigh stress force were researched. The effects of protective agents on glucose utilization rate and ammonia production rate were investigated. It is shown that PEG at the concentration of 0. 04-0. 10 % (W/V)can protect hybridoma cell growth, adding PEG enlarge the glucose utilization rate, but don' t effect ammonia production rate, Adding dextran decrease ammonia production rate, but don' t glucose utilization rate, dextran restrain cell growth, can' t be used as protective agent. Calf serum can protect cell.Cell death kinetics is a one-class reaction. In 250 ml spinner bottle, 37t, 5% cs in RPMI 1640 medium, agitation speed is 80 rpm, cell can' t normally grow, but adding PEG at tha concentration of 0. 1 %in medium, cell can grow normally.