Rapid detection of digoxin in serum samples by molecular imprinted biosensor

LI Min1,ZHANG Juankun1,ZHANG Ziyi2,ZHANG Binbin1,FAN Ting1,WU Ping1(1.Key Laboratory of Industrial Microbiology of the Ministry of Education,College of Bioengineering,Tianjin University of Science and Technology,Tianjin 300457,China;2.Department of Health and Exercise Science,Tianjin University of Sport,Tianjin 300381,China)  

The biosensor was prepared by molecular imprinted technique with digoxin-specific adsorption properties.Firstly the imprinted polymer particles were prepared.Then,particles and the agarose were mixed in proportion.The molecular imprinted polymer film sensor for the digoxin was fixed by the mixture on a glassy carbon electrode(GC).The sensor had specific combination of template molecule-digoxin and its electrochemical signals associated with the template concentration.Thus,it could be used to detect digoxin levels in serum.The molecular imprinted biosensor had advantages of simple,low cost,rapid detection,high specificity,and good stability.The detection limit of the biosensor was 1.28 nmol/L and detection time was 5 min.

【Key Words】： molecular imprinted sensor digoxin polymer serum rapid detection
【Fund】： 科技部资助项目(2009GJA10047);; 天津市科委资助项目(08ZXCXSH053 09ZCZDSF04200)
【CateGory Index】： R285

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