THE PRODUCTION OF WHEAT POLLEN CLONE AND ITS EMBRYOGENESIS
LU YAO-KUI AND CHEN TIAN-ZHU (Northwest Institute of Botany,Wugong,Shaanxi)
This paper deals with the wheatpollen clone with a total of 27 subcul-tures for two years and one month andit now still posseses the capability ofdifferentiation.Throughout the workpollens from hybrid wheat of F_1 [WR10235×(7182-0-11-1×7014 Nd_2)]were used as experimental materialsupplied by our institute.Among allsubculture media used,Murashige andSkoog (MS) agar medium supple-mented with 0.6 mg/l NAA and 0.2mg/l KT seems to be the best one fordifferentiation. After the regenerated clone lostthe capability of differentiation foreight months,it could be recoveredon MS agar medium with the supplement of 0.5 mg/l 2,4-D,2 mg/l KTand 500 mg/l CH.For pretreatment,the clone should be placed in MSliquid medium with 0.6 mg/l 2,4-D,0.2 mg/l NAA,2 mg/l 6-BA and500 mg/l CH for three days beforetransplanting. Cytological observation revealedthat the pollen plants were obtainedfrom wheat pollen calli through twoways:(1) Certain specialized embryo cells can divide successively andthen develop into embryoids whichgrow further into plantlets;(2) Somespecialized epidermal cells of the callican divide continually and then develop into buds and plantlets. Besides the production of embryocells from the epidermal cells and alsoa few layer of cells near the epidermisof the calli,they could also arise evenfrom inner layer of old cells of thecalli in other cases.Morphologicalobservation between the differencesof embryoids and buds are examined.