THE SYNTHESIS OF PROTEIN ENCODED BY COLEI-HHAL FRAGMENT IN A CELL FREE COUPLED TRANSCRIPTION TRANSLATION SYSTEM
CHEN HUI-ZHU (Shanghai Institute of Plant Physiology,Academia Sinica)
In the coupled system for RNAand protein synthesis by using 1mgof S-30 extracts per ml of incubationmixture,it could reduce the backgro-und protein synthesis. Six ColEl-Hhal DNA fragmentswere used as template to direct protein synthesis in lower S-30 extractcouple system. The products were verified by pre-cipitation test with colicin antiserum.