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THE SYNTHESIS OF PROTEIN ENCODED BY COLEI-HHAL FRAGMENT IN A CELL FREE COUPLED TRANSCRIPTION TRANSLATION SYSTEM

CHEN HUI-ZHU (Shanghai Institute of Plant Physiology,Academia Sinica)  
In the coupled system for RNAand protein synthesis by using 1mgof S-30 extracts per ml of incubationmixture,it could reduce the backgro-und protein synthesis. Six ColEl-Hhal DNA fragmentswere used as template to direct protein synthesis in lower S-30 extractcouple system. The products were verified by pre-cipitation test with colicin antiserum.
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