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《Acta Biologiae Experimentalis Sinica》 1984-01
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EXPERIMENTS CONCERNING THE MATURATION RESUMPTION IN BUFO BUFO GARGARIZANS OOCYTE:PROGESTERONE ACTS DIRECTLY ON THE CYTOPLASM

TSO JIA-KE,ZHU GUANG,HSU KUO-GIANG AND WANG YU-LAN (Shanghai Institute of Cell Biology,Academia Sinica)  
Zona pellucida and plasma membrane of the full-grown oocyte of thehibernated common Chinese toad,Bufobufo gargarizans,can be stripped offcompletely after the removal of theovarian membrane and follicular epi-thelium membrane under dissectingmicroscope (Plate Ⅰ,Fig.1—2).Whenthese plasma membrane free oocyteswere embedded in 2% agar blockseparately and immersed in Ringer'ssolution containing progesterone (5μg/ml.),maturation-promoting factor(MPF) was found within the ooplasmsomewhat unexpectedly.Transfer ofthe ooplasm (50 nl.) of these denudedoocytes into the untreated toad oocytethrough microinjection induced theresumption of meiotic division (Fig.1and Table 1) of the later.GVBD,chromosome condensation,first polarbody extrusion and the metaphase figureof the second meiotic division wereevolved normally.If the germinal ve-sicle of the plasma membrane freeoocyte was also removed at the sametime,the maturation promoting effectof progesterone on such denuded oocyte was still the same (Fig.2 and Table2). It was observed that the maturation,promoting activity appeared insome of the denuded oocytes after pro-gesterone treatment for three hours.The longer the hormone treatmenttime,the higher the percentage ofGVBD and normal maturation divisionwere induced in the recipient oocy-tes (Fig.3). During the period of the incubation in Ringer's solution containingprogesterone,no neo-membrane for-mation was observed on the surfaceof the denuded oocytes (Plate Ⅰ,Fig.3).Control oocytes which had re-ceived the ooplasm from the non-treated denuded donors,all posses-sed intact germinal vesicles at anyincubation period (Fig.1—2).Furthermore,a concentration of progesterone hormone which may induce thematuration of the oocytes was notfound in the Ringer's solution aroundthe oocytes. It is evident that the surface membranes (zona pellucida and plasmamembrane),as well as the nucleus ofthe oocyte are dispensable to the progesterone-induced MPF formation.InBufo oocytes,as in Xenopus oocytest,progesterone acts through an initialsite in the cytoplasm.That is to say,the steroid hormone,which is knownto work post-transcriptionally,is capable of regulating oocyte maturation,via an intracellular mechanism.
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【References】
Chinese Journal Full-text Database 2 Hits
1 Gu Quan-bao and Tso Jiake (Shanghai Institute of Cell Biology,Academia sinica);STUDIES ON TOAD OOCYTES MATURATION INDUCED BY MICROINJECTION OF PROGESTERONE-LOADED HUMAN RED BLOOD CELL GHOSTS[J];Acta Biologiae Experimentalis Sinica;1988-03
2 Zhu Guang Hsu Kuo-giang Wang Yi-wen Gu Zheng Tso Jia-ke (Shanghai Institute of Cell Biology,Academia Sinica,Shanghai 200031);EXPERIMENTAL ANALYSIS OF MECHANISM CONCERNING THE ENVIRONMENTAL TEMPERATURE EFFECT ON ACQUISITION OF CAPABILITY IN TOAD OOCYTE TO RESUME MEIOTIC DIVISION[J];Acta Biologiae Experimentalis Sinica;1993-04
【Citations】
Chinese Journal Full-text Database 2 Hits
1 WANG YU-LAN ZHU GUANG CHAI SHI-PING TSO JIA-KE ZHAO JIAN-XING (Shanghai Institute of Cell Biology,Academia Sinica);EXPERIMENTAL ANALYSIS OF EGG MATURATION INDUCED BY PITUITARY EXTRACTS[J];Acta Biologiae Experimentalis Sinica;1982-01
2 KU CHUAN-PAO,HSU KUO-GIANG,LIN XIN-KAI AND WANG YU-LAN (Shanghai Institute of Cell Biology,Academia Sinica);COMPARATIVE ANALYSIS OF PROTEIN COMPONENTS OF PLASMA MEMBRANE,ZONA PELLUCIDA AND VITELLINE ENVELOPE OF BUFO BUFO GARGARIZANS OOCYTE BEFORE AND AFTER MATURATION[J];Acta Biologiae Experimentalis Sinica;1983-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Fresh-water Fisheries Institute of Chehiang Province LIU YUAN-JIE Xu GU-XING YE SHENG-ZHONG;THE INDUCTION OF OVULATION AND MATURATION OF THE OOCYTES OF CTENOPHARYNGODON IDELLUS BY HUMAN CHORIONIC GONADOTROPHIN[J];Journal of Fisheries of China;1966-01
2 Wang Lipin Zhou Xiaofu Xu Hongwei (Slplng Teacher's College);THE DEVELOPMENT OF RESEARCH OF ANUROUS AMPHIBIAN HIBERNATION[J];SONGLIAO JOURNAL;1991-02
3 YAO YONG;GU QUAN-BAO~+;ZHU HUI;BAO YONG-DE;TSO JIA-KE~+ Shanghai Institute of Physiology,~+Shanghai Institute of Cell Biology, Shanghai 200031;A VOLTAGE-DEPENDENT POTASSIUM CHANNEL OF OUTWARD RECTIFIER TYPE IN PLASMA MEMBRANE OF OOCYTE FROM TOAD, BUFO BUFO GARGARIZANS[J];Acta Physiological Sinica;1992-05
4 GU QUAN-BAO;ZHU HUI~+;LI WEI`+;YAO YONG~+;BAO YONG-DE~+;TSO JIA-KE;YANG XIONG-LI~+Shanghai Institute of Cell Biology,Shanghai Joint Laboratory of Life Science,Academia Sinica, Shanghai 200031 ~+Shanghai Institute of Physiology,Shanghai Joint Laboratory of Life Science,Academia Sinica,Shanghai 200031;THE EXPRESSION OF KAINATE AND GABA RECEPTORS IN OOCYTES OF BUFO BUFO GARGARIZANS[J];Acta Physiological Sinica;1992-05
5 TCHOU-SU WANG YU-LAN Institut de Biologie Expérimentale.Academia Sinica.(Shanghai).;éTUDES EXPéRIMENTALES SUR LE R LE DU MUCUS DES OVIDUCTES DANS LA FéCONDATION CHEZ LE CRAPAUD,ET LA CONSIDéRATION GéNéRALE SUR LE MéCANISME DE LA PéNéTRATION SPERMATIQUE[J];;1956-01
6 YUAN CHUN-CHI (Nanking University);INDUCTION OF OVULATION AND MATURATION IN THE TOAD,BUFO BUFO ASIATICUS,WITH EXTRACT OF ANTERIOR LOBES OF BEEF PITUITARY GLANDS[J];;1958-01
7 WANG YING-TIEN(Institute of Experimental Biology, Academia Sinica, Shanghai);INDUCED OVULATION AND MATURATION OF THE OOCYTES WITH HUMAN CHORIONIC GONADOTROPHIN AND STEROID HORMONES IN THE TOAD, BUFO BUFO GARGARIZANS[J];;1963-03
8 TCHOU SU WANG YU-LAN (Institute of Experimental Biology,Academia Sinica,Shanghai);THE DEVELOPMENT OF EGGS OF BUFO BUFO ASIATICUS INSEMINATED AT DIFFERENT STATES OF MATURITY[J];;1964-02
9 WANG YU-LAN ZHU GUANG CHAI SHI-PING TSO JIA-KE ZHAO JIAN-XING (Shanghai Institute of Cell Biology,Academia Sinica);EXPERIMENTAL ANALYSIS OF EGG MATURATION INDUCED BY PITUITARY EXTRACTS[J];Acta Biologiae Experimentalis Sinica;1982-01
10 WANG YU-LAN HSU KUO-GIANG ZHU GUANG (Shanghai Institute of Cell Biology Academia Sinica);MORPHOLOGICAL CHANGES IN THE TOAD OOCYTE ENVELOPES DURING MATURATION AND THEIR RELATIONSHIPS WITH OVULATION[J];Acta Biologiae Experimentalis Sinica;1982-03
【Co-references】
Chinese Journal Full-text Database 7 Hits
1 WANG YU-LAN ZHU GUANG CHAI SHI-PING TSO JIA-KE ZHAO JIAN-XING (Shanghai Institute of Cell Biology,Academia Sinica);EXPERIMENTAL ANALYSIS OF EGG MATURATION INDUCED BY PITUITARY EXTRACTS[J];Acta Biologiae Experimentalis Sinica;1982-01
2 WANG YU-LAN HSU KUO-GIANG ZHU GUANG (Shanghai Institute of Cell Biology Academia Sinica);MORPHOLOGICAL CHANGES IN THE TOAD OOCYTE ENVELOPES DURING MATURATION AND THEIR RELATIONSHIPS WITH OVULATION[J];Acta Biologiae Experimentalis Sinica;1982-03
3 Yang Lian-sheng (Shanghai Institute of Cell Biology,Academia Sinica);CYTOPLASMIC MEMBRANES ARE INVOLVED IN THE REGULATION OF BUFO BUFO GARGARIZANS RESUMING MEIOTIC DIVISION[J];Acta Biologiae Experimentalis Sinica;1987-04
4 Hsu Kuo-giang Zhu Guang Yang Liang-shen and Tso Jia-ke (Shanghai Institute of Cell Biology,Academia Sinica);EFFECT OF ENDOGENOUS cAMP CHANGES ON THE OOCYTE MATURATION OF BUFO BUFO GARGARIZANS[J];Acta Biologiae Experimentalis Sinica;1987-04
5 Zhao Jian-xing,Zhu Guang,Hsu Kuo-gzang,Wang Yu-lan and Tso Jia-ke (Shanghai Institute of Cell Biology,Academia Sinica);IN VITRO STUDIES ON CHANGES OF THE RESPONSE TO PROGESTERONE STIMULATION OF OVARIAN OOCYTES OBTAINED FROM TOADS REARED AT HIGH TEMPERATURE[J];Acta Biologiae Experimentalis Sinica;1988-02
6 Gu Quan-bao,Hsu Kuo-giang and Tso Jia-ke (Shanghai Institute of Cell Biology,Academia Sinica);THE EFFECT OF CALCIUM ON TOAD OOCYTE MATURATION INDUCED BY PROGESTERONE[J];Acta Biologiae Experimentalis Sinica;1988-02
7 Zhao Jian-xing Tso Jia-ke (Shanghai Institute of Cell Biology,Academia Sinica) Shin Yu-liang Wang Wen-ping (Shanghai Institute of Physiology,Academia Sinica);EXPERIMENTAL ANALYSIS OF MEMBRANE POTENTIAL CHANGES DURING MATURATION OF BUFO BUFO GARGARIZANS OOCYTES[J];Acta Biologiae Experimentalis Sinica;1989-03
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 KONG Wei-hua 1,GU Zheng 2,PAN Yong 2,ZUO Jia-ke 2 (1.Life Science College of Shandong Univ.,Jinan 250100,Shandong,China; 2.Shanghai Institute of Planned Parenthood Research,Shanghai 200032,China);CONSTRUCTION OF cDNA LIBRARY OF OOCYTES FROM HIGH TEMPERATURE TREATED TOAD[J];Journal of Shandong University(Natural Science Edition);2001-04
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 WANG YU-LAN ZHU GUANG CHAI SHI-PING TSO JIA-KE ZHAO JIAN-XING (Shanghai Institute of Cell Biology,Academia Sinica);EXPERIMENTAL ANALYSIS OF EGG MATURATION INDUCED BY PITUITARY EXTRACTS[J];Acta Biologiae Experimentalis Sinica;1982-01
2 WANG YU-LAN HSU KUO-GIANG ZHU GUANG (Shanghai Institute of Cell Biology Academia Sinica);MORPHOLOGICAL CHANGES IN THE TOAD OOCYTE ENVELOPES DURING MATURATION AND THEIR RELATIONSHIPS WITH OVULATION[J];Acta Biologiae Experimentalis Sinica;1982-03
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