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STUDY ON RAISING THE REGENERATION RATE OF PROTOPLASTS OF BACILLUS SUBTILIS

GUO DE-DONG,ZHOU DONG-PO,ZHANG LI-QIN,PING WEN-XIANG,CAO ZHONG-JUN,HONG JIAN-ZHONG AND YU AI-HUA.(Biological Department of the Qiqihar Teachers' College) LIU XU-ZHANG (Biological Department of Wuhan University)  
This paper reports the results ofthe effects of types of protoplast solu-tion,its osmotic pressure and pH,the operational temperatures,concen-trations of lysozyme,and bacterialgrowth phases etc.upon protoplastformation viability and the mothodof raising the regeneration rate of pro-toplasts of Bacillus subtilis. The protoplast solution (HM),which was used by Fodor to Bacillusmegaterium was adapted to Bacillussubtilis in our experiment for the firsttime.After the readjusting of the osmotic pressure in the solution,a re-generation rate of 13.3% was ob-tained.Furthermore,a reimprove-ment by adding a suitable amountof the bovine serum albumin into theHM solution,shortening the time oflysozyme action and mixing the sterilized Enterobacter aerogenes with theprotoplasts to the regeneration medium for a plating culture,could increase the regeneration rate furthereven up to 44.47%.Such a resultwould be a piece of valuable information for further research on thefusion of bacterial cells.
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