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《Acta Biologiae Experimentalis Sinica》 1986-01
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IMMUNOFLUORESCENCE STUDY WITH AN AUTOANTINUCLEOLUS ANTISERUM FROM SCLERODERMA PATIENT AS COMPARED WITH SILVER STAINING

Young Young (Shanghai Institute of Cell Biology,Academie Sinica)  
An autoantiserum specific to nucleolusfrom a Scleroderma patient(number 2859)was screened out and used to investigate thenature and distribution pattern of the nuc-leolar antigen(s)by means of indirect im-munofluorescent staining and cytochemicalanalysis,and a comparative study betweenthe antigen(s)and nucleolar argyrophilicprotein was made with special attention.Theresults of cytochemical analysis suggestedthat the nature of the nucleolar antigen(s)is(are)protein(s),not complexed withDNA or RNA,and the antigen(s)is(are)not the argyrophilic protein(s)although thebehaviours of these proteins are very similar.Both the antigen(s)identified by the specificantiserum and those proteins revealed by thesilver staining are represented as granules inthe nucleoli during the interphase,in nuc-leolar organizer regions of metaphasic andanaphasic chromosomes during mitosis.Fur-thermore,both immunofluorescent and silverstaining revealed that the nucleolus-likebodies in micronucleated cells induced bycolchcine treatment consist of nucleolar com-ponents.It was found that the numbers ofnucleolus-like bodies are much more thanthat of NORs possessed by the cell.Thereforethe formation of some nucleolus-like bodiesis independent of NORs.The significance ofthese results was dicussed.
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【References】
Chinese Journal Full-text Database 1 Hits
1 Yang Yang (Shanghai Institute of Cell Biology, Academia Sinica);A NOVEL GROUP OF NUCLEOLAR ORGANIZER REGION ASSOCIATING PROTEINS[J];Acta Biologiae Experimentalis Sinica;1990-03
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Hu Yinghe Liang Hong Jiang Yaoqing (Institute of Genetics, Academia Sinica, Beijing);Studies on Formation of Micronucleoli by Laser Irradiation and Their Cytochemical Analysis[J];Acta Genetica Sinica;1984-05
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 Zhu Jiuming(Institute of Animal and Veterinary Science,Shanghai Academy of Agricultural Science);Applications of laser irradiation in cytology and genetics[J];Laser Journal;1988-01
2 JIA Zhi liang 1, BAO Zhen min 1, PAN Jie 1, WAN Jun fen 1, WANG Ru cai 1 (1 Ocean University of Qingdao, Qingdao 266003, China);The instant detection of live triploid of Pacific oyster (Crassostrea gigas)[J];Acta Oceanologica Sinica;2001-02
【Co-references】
Chinese Journal Full-text Database 2 Hits
1 C.P.CHU AND L.C.SZE Peking Laboratory,Institute of Experimental Biology,Academia Sinica,Peking,China;NORMAL STAGES IN THE DEVELOPMENT OF RANA NIGROMACULATA[J];Acta Anatomica Sinica;1957-01
2 Wang Weizhe;A Modified Primary Monolayer Culture of Embryonic Chicken Leg Chondrocytes in Vitro[J];Chinese Journal of Endemiology;1982-04
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Hu Yinghe Liang Hong (Institute of Genetics, Academia Sinica, Beijing);A Study on the Formation of Micronucleoli by Argon Laser Microirradiation[J];Acta Genetica Sinica;1982-06
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