Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Biologiae Experimentalis Sinica》 1986-01
Add to Favorite Get Latest Update

VIRUS ISOLATION,PURIFICATION AND IDENTIFICATION FROM AN ACUTE INFECTIOUS HAEMORRHAGIC DISEASE OF RABBIT (Oryctolagus cuniculus domestica)

Deng Zhen-wen Li Yon-bao Sun Hsich-chun and Yao Tseng-hsu (Shanghai Institute of Cell Biology,Academia Sinica) Liu Hong-chang Xiao Qing-lin and Mao Shi-hong (Shanghai College of Agriculture)  
Since last year,many rabbit breedingcolonies in different regions in China hadbeen successively attacked with an acute di-sease,characterized by sudden onset,earlydeath,and usually high mortality rate.Ty-pical clinical symptoms of the diseased ani-mals were dull,no appetite and continuousfever.Morbid anatomy showed haemorrhagein liver,kidney,lung and spleen.Observa-tions made previously on ultrathin sectionsof the haemorrhagic tissue under electronmicroscope indicated that numerous virus-likeparticles were present in the cytoplasm ofthe almost disintegrated cells(Fig 1).In thepresent report,virus isolation and purifica-tion were performed from homogenates of rab-bit haemorrhagic liver and kidney,aimingat characterizing the virus-like particles andascertaining the viral etiology of this infec-tious disease. Purified preparation was obtained throughsucrose density gradient centrifugation.Byelectron microscopic examination,a large nu-mber of nonenveloped virus particles,withan outer diameter 34—37 nm and an innerdiameter about 20 nm was observed.The ou-ter surface of the virus was evenly spacedwith knob-like structures.From both thesize and morphological features,the isolatedvirus is very similar to calicivirus(Fig 2).Nucleic acid extracted with phenol/chloro-form from purified virus preparation afterhaving been digested with proteinase K,wasidentified by paper chromatogram method de-scribed by Buchanan(1951).Purified viruspreparation was also stained with acridine ??orange along with those treated by RNase orDNase for differentiating RNA and DNAvirus,according to the procedure described byHsuing(1973).Both results indicated thatthe virus is of RNA type and may be singlystranded.To confirm the etiological relation-ship,we infected two healthy young rabbitsthrough intramuscular injection of suspensionof the purified virus preparation.Typicalclinical sign,as mentioned above,appearedwithin 24 hours,and haemorrhage in tissuesalso manifested after morbid anatomy.In ad-dition,when sera obtained from a few rab-bits,survived after repeated injections withhomogenate made from the haemorrhagic tis-sues and one boosted injection,were addedto the purified virus suspension,obvious ag-glutination of the viral particles occurred.This result further indicated that the survi-ved rabbits had produced antibodies agaist theisolated RNA virus after immunization.Fromthese findings,together with that similarvirus particles were usually found in thehaemorrhagic tissues from diseased rabbits inthe farm,it is evident that the isolated RNAvirus is the pathogen of the acute infectiousdisease of rabbit.In accordance with theclinical and pathological features,we tenta-tively suggest that this newly isolated virusmay be named haemorrhagic fever virus ofrabbit(Oryctolagus cuniculus domestica),be-longing to picornaviridae.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 7 Hits
1 Ji Chuanyi Wang Yongkun (Department of Veterinary Science, Jiangsu Agricultural College);MORPHOLOGY OF RABBIT HEMORRHAGIC DISEASE VIRUS ( RHDV )[J];Chinese Journal of Virology;1990-04
2 Cui Zhizhong Duan Yuyou Wang Yongkun Zhu Guoqiang(Department of Animal Science and Veterinary Medicine of AgriculturalCollege ,Yangzhou University, Yang zhou,225001);COMPARISON OF GENOMES OF CHINESE AND GERMAN STRAINS OF RABBIT HAEMORRHAGIC DISEASE VIRUS[J];CHINESE JOURNAL OF VIROLOGY;1995-03
3 Zheng Hong Zhao Lin Sun Fulin (Wuhan Institute of Virology.Academia Sinica,Wuhan 430071);A NEW VIRUS OF RABBIT——Ⅱ.STUDY ON MORPHOLOGICAL STRUCTURE AND SOME PHYSICOCHEMICAL PROPERTIES OF A STRAIN OF RABBIT HEMORRHAGIC DISEASE VIRUS[J];Acta Microbiologica Sinica;1992-03
4 YAN Qing 1, YAN Jiang hua 1,FANG Ying 1, HUANG Yin yao 2, NI Zi mian 3, CHEN Xin zhong 2 (1. Cancer Res. Center, School of Life Sci., Xiamen Univ., Xiamen 361005, China;2.Xiamen entry exit inspection and quarantine bureau, Xiamen 361005, C;Isolation and Examination of "Mad Swimming Symptom"Virus of Anguilla Anguilla[J];Journal of Xiamen University(Natural Science);2001-05
5 Li Tianxian, Zhao Lin, Feng Feng, Chen Shengliang (Wuhan Institute of Virology,Academia of Sinica,Wuhan 430071);STUDY ON SOME PROPERTIES OF AN ISOLATE OF RHDV[J];CHINESE JOURNAL OF ANIMAL AND VETERINARY SCIENCES;1999-04
6 LI Tianxian; XIONG Kejuan; CHEN Shengliang; CHEN Weimin; ZHAO Lin(Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071);Preservation and Immunization of a RHDV Vaccine Strain[J];CHINESE JOURNAL OF PREVENTIVE VETERINARY MEDICINE;1999-06
7 Liu Xingfa The Military Medical Institute of Lanzhou Military Area;A Slide Hemagglutination Assay for Rapid Detection of Rabbit Hemorrhagic Fever Virus[J];;1989-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Xiang Hong Xu Weiyan Du Nianxing(Department of Veterinary Medicine, Nanjing Agricultural University);DETERMINATION OF BOVINE LEUKEMIA VIRUS INFECTED B LYMPHOCYTES USING B-ROSETTE TEST AND ANTI-BLV 125l-F(ab') 2 AUTORADIOGRAPHY TECHNIQUE[J];Chinese Journal of Virology;1986-01
2 Du Nianxing Xu Weiyan Liu Shengjiang (Division of Veterinary Microbiology, Nanjing Agricultural University, Nanjing);A PRELIMINARY REPORT ON THE IDENTIFNTIFICATION OF A NEW VIRUS --RABBIT HEMORRHAGIC DISEASE VIRUS[J];Chinese Journal of Virology;1986-02
3 Zhou Tong Pu Beiching Nanjing Medical College. The Scientific and Technical Association of Wuxi County.;SOME PHYSICO-CHEMICAL PROPERTIES OF A RABBIT HEMORRHAGIC VIRUS[J];Chinese Journal of Virology;1986-03
4 Shen Juying Peng Baosheng Shen Xueren Peng HaiKung Tsuhsun Fan Yingyuan Yu Ming Chen Yi Xu Quanxing Yang Huiping Chen Jiadi Shanghai Institute of Biochemistry, Academia Sinica.Institute of Animal and Veterinary Sciecnes, Shanghai Academy of Agricultural Sciences.;STUDIES ON VIRAL HEMORRHAGIC DISEASE OF RABBIT Ⅰ.ULTRASTRUCTURAL ALTERATIONS OF LIVER CELLS OF DISEASED RABBITS[J];Chinese Journal of Virology;1986-04
5 Shen Xueren Peng Hai Shen Juying Peng Baozheng Kung Tsuhsun Chen Yi Fan Yingyuan Yu Ming Zhao Hongxing Zhou ZhiaiXu Quanxing Chen Jiadi Shanghai Institute of Biochemistry, Academia Sinica. Institute of Animal and Veterinary Sciences, Shanghai Academy of Agricultural Sciences.;STUDIES ON VIRAL HEMORRHAGIC DISEASE OF RABBIT Ⅱ.PURIFICATION,ELECTRON MICROSCOPY AND NUCLEIC ACID OF RABBIT HEMORRAHAGIC VIRUS[J];Chinese Journal of Virology;1986-04
6 Huang Yinyao; Chen Xiuzhong(Xiamen Animal and Plant Quarantine Service)Yan Jianghua(Cancer Research Centre, Xiamen University);A Investigation on the Epidemiology of Perichareia in Anguilla anguilla[J];FUJIAN JOURNAL OF ANIMAL HUSBANDRY;1997-02
7 Deng Ruitang, Xu Weiyan, Du Nianxing(Section of Veterinary Microbiology, Nanjing Agricultural University)Yang Xicai, and Wu Shi xuan(Institute of Microbiology, Chinese Academy of Science);CHARACTERISTICS OF RABBIT HEMORRHAGIC DISEASE VIRUS[J];Journal of Nanjing Agricultural University;1987-02
8 Yang Xue-lou, Sun Song-bai, Wang Han-zhong, Lou Jing,Huang Wen-ling,Wu Xi-ying, Liu Hong, Waag Xue-lan.(Wuhan Institute of Virology, Academia Sinica,Wuhan);Study on the Morphology and Structure of the Virion of the Rabbit Hemorrhagic Disease Virus(RHDV)[J];Virologica Sinica;1989-02
9 Wang Yongkun, Ji Chuanyi, Zhou Yangsheng, Sun Huaichang, Tian Huifang, Xu Yimin, Wang Baoan, Huo Jinfu, Li Junbao Gao Jinliang, Shao Tiannao, Yu Hongli (Jiangsu Agricultural College, Yangzhou, Jiangsu Province);STUDY ON A NEW VIRUS INFECION IN RABBITS——RABBIT PEST[J];Scientia Agricultura Sinica;1988-05
10 Du Nianxing Xu Weiyan Liu Shengjiang Xu Funan Yu yunru Li Rujian (Nanjing Agricultural University, Nanjing, Jiangsu Province);STUDIES ON RABBIT HAEMORRHAGIC DISEASE[J];Scientia Agricultura Sinica;1991-01
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 CHEN Sheng-liang,LI Tian-xian,ZHANG Zhong,YANG Bao-yu (Wuhan Institute of virology,Academia Sinica, Wuhan,Hubei,430071,China);The Effect of Preservation and Inactivated Vaccine of RHDV[J];Progress In Veterinary Medicine;2002-05
2 LU Hong-da1,DUAN Qiu-ming2,ZHU Guang-lai1,ZHANG Lian-yi1(1.College of Fisheries and Life,Shanghai Ocean University,Shanghai 200090,China;2.Agriculture Bureau of Shicheng County,Jiangxi Province,Shicheng 342700,China);Gill histopathology of European eel(Anguilla anguilla) with Pseudodactylogrus bini disease[J];Journal of Fisheries of China;2008-05
3 YANG Long-sheng~1,XUE Jia-bin~2,DING Guo-ming~3,XU Wei-zhong~2,CHEN Xing-xiang~2,KONG Xiang-feng~1 and HU Yuan-liang ~(1*)(1.Nanjing Agriculture University,Nanjing 210095,China;2.Institute of Veterinary Science of Jiangsu Academy of Agriculture Science,Nanjing 210014,China;3.Agricuture Service Centre of Xinlian of Tongzhou,Tongzhou Jiangsu,China);The Comparison of Some Methods of Purifying RHDV[J];Acta Agriculturae Boreali-Occidentalis Sinica;2006-02
4 Liu Yongde, Wang Yi, Xu Jun, Shi Caipei, Chen Zhilong ( Department of Animal Science, Shanghai Agricultural College );STUDY ON COMPARISON OF THREE RAPID DIAGOSIC METHODS FOR RABBIT HEMORRHAGIC DISEASE[J];Chinese Journal of Animal and Veterinary Sciences;1992-04
5 YAN Wei-wei1, CUI Zi-zhong2**, WANG Yong-kun1 (1. Animal Science and Veterinary Medicine College Yangzhou University, Yangzhou, 225009, China; 2. Animal Science College Shandong Agricultural University, Taian, 271018, China );Cloning and Sequencing of the Capsid Protein Gene of Rabbit hemorrhagic disease virus Isolate WX84[J];Virologica Sinica;2003-02
6 SHEN Zhi-yi LI Li-ping DU Ke-qin ZHAO Wen-shuang LI Ping-an (Inner Mongolia Agricultural University,Huhhot,010018,China);Isolation and Characterization of Rabbit Hemorrhagic Disease Virus in Inner Mongolian Region[J];China Animal Quarantine;2003-05
7 LIU Huai-ran,GUAN Yun-tao,LI Chang-wen,ZHANG Ling,JIANG Qian,CHEN Hong-yan,QU Lian-dong~*(Heilongjiang Provincial Quality Monitoring Center for Laboratory Animals,Harbin Veterinary Research Institute of CAAS.Harbin 150001,China);Preparation and Preliminary Application of Indirect ELISA Kits for Rabbit Hemorrhagic Disease Antibody Detection[J];Acta Laboratorium Animalis Scientia Sinica;2006-01
8 ZENG Qunhui,ZHU Guoyu,SUO Zhen,TIAN Fayi,XIE Xiuguo,GAO Juntao,HOU Dianlei (The Department of Animal Husbandry and Veterinary,Tibet Agricultural College,Bayi,Tibet 860000);Symptom of Rabbits Heamorrhagic Disease Virus in Tibet ——The Virus Identification and Experiment of Vaccine Immune Effect[J];CHINESE JOURNAL OF PREVENTIVE VETERINARY MEDICINE;2000-02
9 LIU Huai-ran,CHEN Hong-yan *,GUAN Yun-tao,WANG Yun-feng ①,LI Chang-wen,XIA Chang-you,ZHANG Li-cheng ② (Harbin Veterinary Research Institute of CAAS,Heilongjiang provincial center for quality monitoring of laboratory animals,Harbin 150001, China);Cloning and Sequence Analysis for Capsid Protein VP60 Gene of Rabbit Hemorrhagic Disease Virus[J];Chinese Journal of Preventive Veterinary Medicine;2002-05
10 Wang Keling Lang Limin Guo Xiuqi Wang Sibao Wei Chengbin (Institute of Animal Husbandry and Veterinary Sciences, Henan Academy of Agricultural Sciences,Zhengzhou 450002);Studies on Human Red Blood Cells Hemagglutination Pedigree with Rabbit Hemorrhagic Disease(RHD)[J];CHINESE JOURNAL OF RABBIT FARMING;1999-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved