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《Acta Biologiae Experimentalis Sinica》 1987-01
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INHIBITION AND REVERSION OF EPSTEIN-BARR VIRUS DNA REPLICATION IN H_(18) CELL LINE

Zhao De-biao (Shanghai Institute of Cell Biology.Academia Sinica)  
EBV genome equivalents per H~(18) cellwere estimated as about 207 by cell spothybridization with EBV DNA BamHI-Wfragment as probe and dropped abruptly withthe treatment of PAA.By day 11 after ad-ding PAA at a concentration of 75 Y/ml,theaverage number of EBV genome equivalentper cell decreased to 17,only about 8% ofthe untreated cell (Fig.3).and then main-tained at this level constantly under conti-nuous treatment,while the synthesis of EBVVCA was completely inhibited,but that ofEA was not affected.Removal of the drugat day 3 allowed a recovery to 29% of thegenome equivalent of the control value and.at day 40 to 90%.Whereas retreatmentwith n-butyrate after removal of PAA inter-fered with such recovery significantly (Fig.5).It is commonly believed that the replicationof EBV episome is mediated by host-cell DNApolymerase and the synthesis of viral linearDNA is mediated by the virus-induced PAA-sensitive DNA polymerase.Since n-butyratecan effectively prevent the host DNA repli-cation apparatus,therefore our results suggestthat both the host and virus-induced DNApolymerase might play a role in the recoveryof EBV DNA content in H~(18) cells after re-moval of PAA treatment.
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