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《Acta Biologiae Experimentalis Sinica》 1987-01
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PNA-GLYCOPROTEIN PATTERNS OF CHICK RETINA DURING EMBRYO DEVELOPMENT

Liu Li and Song Qiu-bao (Shanghai Institute of Cell Biology,Acadeinia Sinica)  
In the previous study,by means of FITCcoupled peanut agglutinin (FITC-PNA) tech-nique the temporal and spatial patterns ofthe developmental chick retina were revealed.It has been found that the PNA binding si-tes appeared first in inner plexiform layer(IPL)of 7-day-old embryo (E7) and outerplexiform layer (OPL) of E 9 during earlydevelopmental stages of the embryo.The to-tal protein patterns were revealed by two-dimensional microgel-electrophoresis,and thePNA binding glycoprotein patterns by theimmuno-blotting technique.With electropho-resis method,quantitative as well as qualita-tive change in the total protein of the retinaof E4-E16 were observed (Plate I.Fig.1—4).The specific spots of glycoproteins havenot been found in the retina of E4,whilethey appeared in E 6 and then increased con-tinuously.The propotion of spot number ofthe glycoproteins in total proteins was higherin E 6 than E 8—E16.The most of increa-sed glycoproteins were of middle molecularweights and in the neutral pH-range.Thepropotion of glycoprotein in total protein ofthe optic nerve was about 2 folds of that ofthe retina at the same developmental stage(Plate II,Fig.5—8,) implying that the gly-coproteins mainly located on the cell surface.In addition,the total protein patterns in theretina were more similar to those in the tec-tum than to those in the telencephalon. These results indicate that the PNA-bin-ding glycoprotein patterns are in closeconnection with the developmental stage.Theglycoproteins may play an important role ininteraction between cells.Some function ofthe glycoproteins,such as cell adhesion andretino-tectum projection were discussed.
【Fund】: 中国科学院科学基金资助的课题(84科基金生准字第479号)
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