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《Acta Biologiae Experimentalis Sinica》 1988-01
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Qi Hui-lin (Shanghai Institute of Cell Biology,Academia Sinica)  
In this paper interphase nuclear MTOC is characterized by using the indirect immu-nofluorescent staining with monospecific 6s tubu lin antibodies.Microtubules grow only from a few nuclei in the population of isolated nuclei after the nuclei are incubated with 6s tubu-lin.It is shown that nuclei which can grow microtubules in the population of isolated nuclei are lack of,or with damaged,nuclear membrane,as indicated by the experiments of the demembraned nuclei with Triton X-100 or lysolecithin.All the isolated nuclei when demembraned with these agents,grow microtubules.When these nuclei with microtu- bules are depolymerized,immunofluorescent particles can still be observed.The number of these particles in the nuclei is larger than that of the chromosomes.This suggests that the numbers of the nuclear MTOCs is larger than that of the spindle fibre attachments (SFAs).It is possible that interphase nuclear MTOCs consists of SFAs and some other ch-romatin particles.And they have affinity with 6s tubulin,since when the demembran-ed nuclei were incubated with the mixture of 6s tubulin and colchicine,immunofluores-cent particles could be observed.
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