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《Acta Biologiae Experimentalis Sinica》 1988-01
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THE REPRODUCTION OF SWINE VESICULAR DISEASE VIRUS IN MICROCELLS AND MICROCYTOPLASTS

Li Wei-gang Chen Li-hua and Zhai Zhong-he (Department of Biology,Peking University)  
We had studied the reproduction of swine vesicular disease virus (SVDV) in the micro-cells and the microcytoplasts prepared from IB-RS-2 cells.More than 70% of the micro-cells of IB-RS-2 cells were alive in the nor-mal culture for 60 hours,while the micro-cells infected with SVDV were almost broken away from the substrate in 5 hours after in-fection.The infective progeny virus was de-tected on the infected microcells. The microcytoplasts infected with SVDV were observed with SEM,The CPE of the microcytoplasts appeared in 4 hours after in- fection.In the experiment using microscopic autoradiography,we found that the microcy-toplasts infected with SVDV were labled by ~3H-uridine,but the uninfected were not,in-dicating that SVDV RNA were replicated in the microcytoplasts.The infective progeny virus were produced in the microcytoplasts in-fected with SVDV for 6 hours.The titer of viruses from microcytoplasts was lower than that of viruses reproduced in the intact cells.The above results suggest that SVDV were able to infect and replicate in microcytoplasts and produce the infective progeny virus.
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