Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

LIPOSOME-MEDIATED DELIVERY OF CALCEIN INTO CUCUMBER SUSPENTION PROTOPLASTS

Tang Hui-xian Jia Shi-rong (Biotechnology Research Center,Chinese Academy of Agricultural Sciences) Yen Chang-cheng Zhao Nan-ming (Department of Biological Science and Biotechnology,Tsinghua University)  
Liposome-encapsulated calcein prepared by 'dehydration-rehydration method' was successfully introduced into cucumber suspen-sion protoplasts by means of PEG-induced fusion or liposome/protoplast co-cultivation.By PEG treatment,the percentage of cells with liposome-mediated delivery of calcein was around 80-90%.among which 50-60% cells showed strong,even distributing fluore-scence.In the case of liposome/protoplast co-incubation (half an hour),the percentage of fluorescent cells reached more than 95%.However,the fluorescence was relatively weak and dot-distributed within cells.The liposo-me was broken after 3-4 days in culture so that the dotted fluorescence became evenly distributed.Cucumber protoplasts uptaking liposomes successively underwent sustained cell division,formed callus and embryoids which eventually regenerated into shoots and roots.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved