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STUDIES ON TOAD OOCYTES MATURATION INDUCED BY MICROINJECTION OF PROGESTERONE-LOADED HUMAN RED BLOOD CELL GHOSTS

Gu Quan-bao and Tso Jiake (Shanghai Institute of Cell Biology,Academia sinica)  
It was found that the meiotic division of full-grown oocytes of Bufo bufo gargari-zans could be induced by microinjection of aqueous progesterone-loaded human red blood cell ghosts (Fig,1,A).Neither the aqueous solution of progesterone nor the red blood cell ghost itself could induce the resumption of meiotic division,when they were microinjected into oocytes respectively (Fig.1,B,C and D).The effects on the induction of oocyte meiotic division of chemical or enzymatic pre-treatment of the red blood cell membrane were analysed:no meiotic division could be resu- med by microinjecting the progesterone-loaded ghosts whose membrane had been pretreated with phloretin (200 !M) (Fig.2,A) or pho-spholipase A_2 (Fig.3,C); but the meiotic di-vision still can be induced by microinjecting the progesterone-loaded ghosts pretreated with pronase E (Fig.3,B).All these results in-dicated above reveal that phospholipids of me-mbrane system are necessary for progesterone to avoid any enzymatic modification in the oocyte and to realize its normal transmem-brane signal in the resumption course of meio-tic division.
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