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《Acta Biologiae Experimentalis Sinica》 1989-01
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A PRIMARY STUDY ON THE SUBSTANCE IN GOLGI APPARATUS IN THE GLANDULAR HAIR CELLS OF BRASENIA SCHREBEI

Wang Wen (Biology Department of Nanjing Normal University)  
In conventional preparation of biologi-cal specimen without any special treating,thesaccules and vacuoles of Golgi apparatus al-ways show electron lucent by transmissionelectron microscope (TEM).But a high ele-ctron dense substance has been found in thesaccules and vacuoles of Golgi apparatus inthe glandular hair cells of Brasenia schrebei(Fig.1).The substance is often in waveshape in the saccules and there are somefine fibres around it (Fig.2). The substance shows positive reactionwhen the ultrathin section is treated withperiodic acid-silver nitrate-methenamine stainby polysaccharide identifing method (Fig.4).This indicates that the electron dense subs-tance contains a great amount of polysac-charide. The polysaccharide in the sections ofOsO_4-fixed tissue has been shown to reducesilver nitrate-methenamine solution even inthe absence of a preliminary oxidation withperiodic acid (Fig.5). The sections of OsO_4-fixed tissue withoutany staining show the staining function ofOsO_4 only.In this condition the wave shapesubstance also can be seen by TEM (Fig.3).This demonstrates that OsO_4 is responsive forstaining polysaccharide.
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【References】
Chinese Journal Full-text Database 2 Hits
1 Wang Wen (Department of Biology);The Electron Microscopic Technique of Displaying Substances in Vacuuoles of Plant Cells by Prolonged OsO_4 Fixation Method[J];Journal of Nanjing Normal University(Natural Science Edition);1993-02
2 XU Guang-Hui SUN Wen-Yun JIANG Feng MA Xiao-Hang (Institute of Microbiology Zhejiang, Hanzhou, 310012);STUDIES ON ECOLOGICAL DISTRIBUTION OF MICROORGANISM ON THE SURFACE OF JUNSAI LEAVES AND ON FACTORS AFFECTING POLYSACCHARIDES FERMENTATION BY MUCILAGE-PRODUCING BACTERIA STRAIN 5242[J];Wuyi Science Journal;1992-00
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 Wang Wen (Biology Department of Nanjing Normal University);The Utilization of the Electron Microscopic Cytochemical Technique of the Silver Methenamine for Detecting Polysaccaride[J];Journal of Chinese Electron Microscopy Society;1989-03
2 Wang wen (Biology Department of Nanjing Normal University);A CYTOCHEMICAL ELECTRON MICROSCOPY FOR SHOWING PROTEOGLYCAN GRANULES IN THE PITUITARY CELL OF FISH[J];Acta Hydrobiologica Sinica;1989-03
3 Wang Shuru, Xia Erning and Zhou Lan (Division of Biochemistry);EXTRACTION, ISOLATION AND SOME BIOLOGICAL ACTIVITIES OF POLYSACCHARIDE OF BRASENIA SCHREBERI[J];Journal of China Pharmaceutical University;1987-03
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