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《Acta Biologiae Experimentalis Sinica》 1993-01
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PLANT REGENERATION FROM MESOPHYLL PROTOPLASTS OF ASTRAGALUS MELILOTOIDES

Zhang Xiang-qi An Li-jia Xiang Wei Zhang Hong-chuan (Department of Biology.Liaoning Normal (?)niversity.Dalian 116022  
Mesophyll protoplasts were isotaledfrom top and basal leaves of regeneratedshoots derived from hypocotyles of Astra-galus melilotoides.The protoplasts from topleaves cultured in P2 medium(K8P me-dium with 1.0mg/L 2,4-D,0.2mg/LNAA and 0.7mg/L 6-BA)showed highercell division frequency(48.9%)and mi-crocallus formation frequency(321 piecesml of protoplast suspension),and the lat-tet was strongly reduced when the proto-plasts were cultured in media with higheror lower concentrations of 2,4-D.Theprotoplasts from basal leaves rarely under-went division,and no callus was obtain-ed.The protoplast-derived microcalli weretransferred to solid media(G1.G2 andG4)or liquid medium(L1)for furtherproliferation.Under these conditions,themicrocalli continued to grow vigrously.After 2—3 weeks,the calli were transfer-red to differentiation media(D1 D4).On which,10(?) of the calli producedshoots,and the shoots rooted by tranfer torooting medium(R).Now,a large num-ber of reg(?)n(?)reted plantlets were obtainedand some of them survived after transplan-tation into soil.
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