THE CHARACTERISTICS OF MONOCLONAL ANTIBODIES AND THEIR ANTIGENS ASSOCIATED WITH HUMAN SPERM ACROSOME REACTION Ⅱ Effects of the monocional antibodies on sperm function,sperm antigen localization and immunoblotting
Wang Yun-mei Ben Kun-long Cao Xiao-mei (Kunming Institute of Zoology,Academia sinica.Kunming 650107
By using mieroassay,the strong hu-man sperm agglutination were caused by 9of 23 monoclonal antibodies(McAbs)ob-tained by immunization with fresh humanacrosome-reacted(AR)sperm,which wasinduced by ionophore A 23187,but no oneof them demonstrated the ability to immo-bilize human sperm.In contrast to moststudies reported by other laboratories,theantigens recognized by most of McAbs inthe present study were located in equato-rial segment and mi(?)piece of human sperm,and no one in aerosome region of humansperm.The binding pattern of some McAbson non-treated and AR sperm was foundto be different.The human sperm proteinsranged in the size of 16-116 kDa were de-monstrated to be (?)aeted with 9 of 23 Mc-Abs by immunoblotting.Moreover,thecross (?)etion of 23 McAbs with mouse andtree shrew sperm was det(?)ted by indirectimmunofluores(?)nt assay.These results sug-gest that AR sperm posses(?)s pe(?)uliar im-munogenic properties.